Our genomes are mostly made up of repetitive 'junk DNA'derived from insertions of sequences through RNA intermediates. Our group has developed methods to identify polymorphic insertions of these understudied sequences, demonstrated they are major sources of structural variation our genome, and found they occur frequently in LD with trait associated SNPs identified by cancer genome wide association study (GWAS). Experiments by others and characterizations of the non-random distribution of mobile DNAs in our genome indicate they have significant potential to effect gene function. The overarching hypothesis of this proposal is that a subset of common mobile DNA insertions predispose to common cancer development. Our three part approach to test this hypothesis will include: (i.) identification of RIPs with potential roles in neoplasia by locating those in the vicnity of regions implicated in disease risk by GWAS;(ii.) determining which of these RIPs may reasonably account for cancer risk by analyses of area linkage and RIP genotype imputing in clinical samples;and (iii.) investigating effects of transposon polymorphisms on transcript expression levels and structure. Hematopoietic malignancies will receive special priority in these studies, and clinical samples from patients with leukemias, lymphomas, and related disordered will be used for a directed RIP discovery effort and for evaluating mechanisms of gene expression effects.
The purpose of the proposed studies is to test the hypothesis that inherited retrotransposon insertion polymorphisms (RIPs) predispose carriers to neoplasias. We will discover potentially relevant RIPs, ascertain which candidates associate with disease risk as appreciated by genome-wide association studies (GWAS), and determine the biologic basis of such effects by gene expression studies.
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|Tang, Zuojian; Steranka, Jared P; Ma, Sisi et al. (2017) Human transposon insertion profiling: Analysis, visualization and identification of somatic LINE-1 insertions in ovarian cancer. Proc Natl Acad Sci U S A 114:E733-E740|
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|Criscione, Steven W; Theodosakis, Nicholas; Micevic, Goran et al. (2016) Genome-wide characterization of human L1 antisense promoter-driven transcripts. BMC Genomics 17:463|
|Achanta, Pragathi; Steranka, Jared P; Tang, Zuojian et al. (2016) Somatic retrotransposition is infrequent in glioblastomas. Mob DNA 7:22|
|Doucet-O'Hare, Tara T; Sharma, Reema; Rodi?, Nemanja et al. (2016) Somatically Acquired LINE-1 Insertions in Normal Esophagus Undergo Clonal Expansion in Esophageal Squamous Cell Carcinoma. Hum Mutat 37:942-54|
|Sharma, Reema; Rodi?, Nemanja; Burns, Kathleen H et al. (2016) Immunodetection of Human LINE-1 Expression in Cultured Cells and Human Tissues. Methods Mol Biol 1400:261-80|
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