Recent results have led many to propose a microenvironment-dependent model for initiation ofmigratory and disseminating tumor cell behavior at both the primary tumor and within targetorgans that is not stably specified by genetic mutation and that is transient in time and space.This view is called the microenvironment model of metastasis. The testing of this model hasbeen hampered in part by the lack of high-resolution in vivo microscopy methods andgenetically-encoded fluorescent probes for tumor deep-tissue imaging that allow definitiveidentification of the microenvironments involved in initiating the migratory and disseminatingtumor cell phenotype. Equally problematic are the limitations of standard analyses of expressionprofiles. Standard analysis of expression profiles in cancer involves identifying consistently up-and down- regulated genes. While these techniques are likely to identify sets of genes directlywithin affected networks, our previous theoretical results have shown that major perturbations(of which cancer is one) cause expression changes far beyond the pathway involved. Crucially,these more distant changes will be highly variable depending on the genetic background, thustumor expression profiles are expected to be greatly dissimilar between individuals. Using thishypothesis we propose a novel systems-level analysis of cancer (SLAC), which identifies keygenes based upon increase in expression variability, and which in turn offers the possibility ofdiscovering highly non-intuitive pathway interactions connected with microenvironmentregulation of breast cancer progression. By combining the multiphoton high-resolutionmicroscopy having the wide range of excitation wavelengths with the proposed multicolor far-redfluorescent probes as versatile as conventional GFP we will advance deep-tissue cell labelingand imaging of tumor cells dynamics in vivo. This approach will make possible the intravitalimaging of simultaneously up to six genetically-encoded colors in tumor studies. This in turn willprovide a way to discriminate and subsequently isolate the tumor cells of multiple metastaticphenotypes based on the fluorescent color-encoded expression patterns. By correlating thebehavior and fate of migrating and disseminating tumor cells obtained by the multiphotonimaging at a single-cell level with SLAC analysis of expression profiles of these cells, we willidentify the key genes driving tumor cell behaviors involved in metastasis such as cell migrationand dissemination.

Public Health Relevance

We will use new technologies for the high-resolution multiphoton microscopy at a single-celllevel in breast tumors combined with development of the advanced genetically-encodedfluorescent probes with for multicolor deep-tissue imaging and a novel Systems-Level Analysisof Cancer (SLAC) methodology to define mechanisms responsible for the spread of breasttumor cells from the primary tumor.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA164468-05
Application #
8911788
Study Section
Special Emphasis Panel (ZCA1-SRLB-9 (O1))
Program Officer
Couch, Jennifer A
Project Start
2011-09-21
Project End
2016-07-31
Budget Start
2015-08-01
Budget End
2016-07-31
Support Year
5
Fiscal Year
2015
Total Cost
$753,636
Indirect Cost
$302,357
Name
Albert Einstein College of Medicine
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
110521739
City
Bronx
State
NY
Country
United States
Zip Code
10461
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