The goal of the research is to use a pump-probe microscopy system to find statistically significant variations between melanomas that did and did not develop metastases. This information will be used to develop automated classification algorithms to find robust molecular and structural features with strong predictive power. These insights will enable a retrospective study of melanoma and sentinel node biopsies, that can be used to validate the findings. By the end of the project, it is expected that there will be statistically significant evidence that pump-probe microscopy can substantially improve the diagnostic accuracy of histopathology.

Public Health Relevance

We propose a novel imaging technology which can image specific cancer biomarkers in developing skin lesions. This approach could reduce the number of false negative diagnoses in thin (less than 1mm) melanomas that uncharacteristically metastasize. Additionally, this approach could eliminate thousands of false positive melanoma diagnoses per year, and save many millions of dollars in healthcare costs.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
1R01CA166555-01A1
Application #
8437772
Study Section
Medical Imaging Study Section (MEDI)
Program Officer
Ossandon, Miguel
Project Start
2013-01-02
Project End
2017-12-31
Budget Start
2013-01-02
Budget End
2013-12-31
Support Year
1
Fiscal Year
2013
Total Cost
$321,376
Indirect Cost
$113,876
Name
Duke University
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
044387793
City
Durham
State
NC
Country
United States
Zip Code
27705
Robles, Francisco E; Deb, Sanghamitra; Fischer, Martin C et al. (2017) Label-Free Imaging of Female Genital Tract Melanocytic Lesions With Pump-Probe Microscopy: A Promising Diagnostic Tool. J Low Genit Tract Dis 21:137-144
Wilson, Jesse W; Robles, Francisco E; Deb, Sanghamitra et al. (2017) Comparison of pump-probe and hyperspectral imaging in unstained histology sections of pigmented lesions. Biomed Opt Express 8:3882-3890
Robles, Francisco E; Zhou, Kevin C; Fischer, Martin C et al. (2017) Stimulated Raman scattering spectroscopic optical coherence tomography. Optica 4:243-246
Fischer, Martin C; Wilson, Jesse W; Robles, Francisco E et al. (2016) Invited Review Article: Pump-probe microscopy. Rev Sci Instrum 87:031101
Robles, Francisco E; Fischer, Martin C; Warren, Warren S (2016) Dispersion-based stimulated Raman scattering spectroscopy, holography, and optical coherence tomography. Opt Express 24:485-98
Thompson, Andrew; Robles, Francisco E; Wilson, Jesse W et al. (2016) Dual-wavelength pump-probe microscopy analysis of melanin composition. Sci Rep 6:36871
Wilson, Jesse W; Degan, Simone; Gainey, Christina S et al. (2015) Comparing in vivo pump-probe and multiphoton fluorescence microscopy of melanoma and pigmented lesions. J Biomed Opt 20:051012
Robles, Francisco E; Deb, Sanghamitra; Wilson, Jesse W et al. (2015) Pump-probe imaging of pigmented cutaneous melanoma primary lesions gives insight into metastatic potential. Biomed Opt Express 6:3631-45
Wilson, Jesse W; Park, Jong Kang; Warren, Warren S et al. (2015) Flexible digital signal processing architecture for narrowband and spread-spectrum lock-in detection in multiphoton microscopy and time-resolved spectroscopy. Rev Sci Instrum 86:033707
Simpson, Mary Jane; Glass, Keely E; Wilson, Jesse W et al. (2014) Correction to ""Pump-Probe Microscopic Imaging of Jurassic-Aged Eumelanin"". J Phys Chem Lett 5:946

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