The goal of the research is to use a pump-probe microscopy system to find statistically significant variations between melanomas that did and did not develop metastases. This information will be used to develop automated classification algorithms to find robust molecular and structural features with strong predictive power. These insights will enable a retrospective study of melanoma and sentinel node biopsies, that can be used to validate the findings. By the end of the project, it is expected that there will be statistically significant evidence that pump-probe microscopy can substantially improve the diagnostic accuracy of histopathology.

Public Health Relevance

We propose a novel imaging technology which can image specific cancer biomarkers in developing skin lesions. This approach could reduce the number of false negative diagnoses in thin (less than 1mm) melanomas that uncharacteristically metastasize. Additionally, this approach could eliminate thousands of false positive melanoma diagnoses per year, and save many millions of dollars in healthcare costs.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA166555-02
Application #
8601919
Study Section
Medical Imaging Study Section (MEDI)
Program Officer
Ossandon, Miguel
Project Start
2013-01-02
Project End
2017-12-31
Budget Start
2014-01-01
Budget End
2014-12-31
Support Year
2
Fiscal Year
2014
Total Cost
$289,239
Indirect Cost
$102,489
Name
Duke University
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
044387793
City
Durham
State
NC
Country
United States
Zip Code
27705
Simpson, Mary Jane; Wilson, Jesse W; Robles, Francisco E et al. (2014) Near-infrared excited state dynamics of melanins: the effects of iron content, photo-damage, chemical oxidation, and aggregate size. J Phys Chem A 118:993-1003
Park, Jong Kang; Fischer, Martin C; Susumu, Kimihiro et al. (2014) Femtosecond pulse train shaping improves two-photon excited fluorescence measurements. Opt Lett 39:5606-9
Robles, Francisco E; Fischer, Martin C; Warren, Warren S (2014) Femtosecond pulse shaping enables detection of optical Kerr-effect (OKE) dynamics for molecular imaging. Opt Lett 39:4788-91
Simpson, Mary Jane; Glass, Keely E; Wilson, Jesse W et al. (2013) Pump-Probe Microscopic Imaging of Jurassic-Aged Eumelanin. J Phys Chem Lett 4:1924-1927
Simpson, Mary Jane; Wilson, Jesse W; Phipps, M Anthony et al. (2013) Nonlinear microscopy of eumelanin and pheomelanin with subcellular resolution. J Invest Dermatol 133:1822-6
Wilson, Jesse W; Vajzovic, Lejla; Robles, Francisco E et al. (2013) Imaging microscopic pigment chemistry in conjunctival melanocytic lesions using pump-probe laser microscopy. Invest Ophthalmol Vis Sci 54:6867-76
Robles, Francisco E; Samineni, Prathyush; Wilson, Jesse W et al. (2013) Pump-probe nonlinear phase dispersion spectroscopy. Opt Express 21:9353-64