The goal of the research is to use a pump-probe microscopy system to find statistically significant variations between melanomas that did and did not develop metastases. This information will be used to develop automated classification algorithms to find robust molecular and structural features with strong predictive power. These insights will enable a retrospective study of melanoma and sentinel node biopsies, that can be used to validate the findings. By the end of the project, it is expected that there will be statistically significant evidence that pump-probe microscopy can substantially improve the diagnostic accuracy of histopathology.

Public Health Relevance

We propose a novel imaging technology which can image specific cancer biomarkers in developing skin lesions. This approach could reduce the number of false negative diagnoses in thin (less than 1mm) melanomas that uncharacteristically metastasize. Additionally, this approach could eliminate thousands of false positive melanoma diagnoses per year, and save many millions of dollars in healthcare costs.

Agency
National Institute of Health (NIH)
Type
Research Project (R01)
Project #
5R01CA166555-05
Application #
9189685
Study Section
Medical Imaging Study Section (MEDI)
Program Officer
Ossandon, Miguel
Project Start
Project End
Budget Start
Budget End
Support Year
5
Fiscal Year
2017
Total Cost
Indirect Cost
Name
Duke University
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
044387793
City
Durham
State
NC
Country
United States
Zip Code
27705
Fischer, Martin C; Wilson, Jesse W; Robles, Francisco E et al. (2016) Invited Review Article: Pump-probe microscopy. Rev Sci Instrum 87:031101
Thompson, Andrew; Robles, Francisco E; Wilson, Jesse W et al. (2016) Dual-wavelength pump-probe microscopy analysis of melanin composition. Sci Rep 6:36871
Robles, Francisco E; Fischer, Martin C; Warren, Warren S (2016) Dispersion-based stimulated Raman scattering spectroscopy, holography, and optical coherence tomography. Opt Express 24:485-98
Robles, Francisco E; Deb, Sanghamitra; Wilson, Jesse W et al. (2015) Pump-probe imaging of pigmented cutaneous melanoma primary lesions gives insight into metastatic potential. Biomed Opt Express 6:3631-45
Wilson, Jesse W; Park, Jong Kang; Warren, Warren S et al. (2015) Flexible digital signal processing architecture for narrowband and spread-spectrum lock-in detection in multiphoton microscopy and time-resolved spectroscopy. Rev Sci Instrum 86:033707
Wilson, Jesse W; Degan, Simone; Gainey, Christina S et al. (2015) Comparing in vivo pump-probe and multiphoton fluorescence microscopy of melanoma and pigmented lesions. J Biomed Opt 20:051012
Park, Jong Kang; Fischer, Martin C; Susumu, Kimihiro et al. (2014) Femtosecond pulse train shaping improves two-photon excited fluorescence measurements. Opt Lett 39:5606-9
Simpson, Mary Jane; Wilson, Jesse W; Robles, Francisco E et al. (2014) Near-infrared excited state dynamics of melanins: the effects of iron content, photo-damage, chemical oxidation, and aggregate size. J Phys Chem A 118:993-1003
Simpson, Mary Jane; Glass, Keely E; Wilson, Jesse W et al. (2014) Correction to "Pump-Probe Microscopic Imaging of Jurassic-Aged Eumelanin". J Phys Chem Lett 5:946
Robles, Francisco E; Fischer, Martin C; Warren, Warren S (2014) Femtosecond pulse shaping enables detection of optical Kerr-effect (OKE) dynamics for molecular imaging. Opt Lett 39:4788-91

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