(PQA4) Point-of-Care Test of Exposure to Hepatocellular Carcinoma Risk by V-Chip Underlying mechanisms for synergisms of HCC risks may provide efficient and accurate HCC prevention strategies for high-risk individuals. People exposed to environmental or life-style risk factors simultaneously exhibit the increased incidence of HCC development. However, most of the burden of HCC is associated with the group of people with low income, with the highest incidence rates reported in regions where infection with hepatitis B virus (HBV) is endemic. Therefore, developing an accurate, quantitative, and also low-cost technology for the rapid detection of biomarkers is required for HCC risk assessment. Herein, we propose an ELISA-based new technology platform, the multiplexed volumetric bar-chart chip (V- Chip), to detect biomarkers in blood samples. The proposed V-Chip is based on microfluidics technology. The integrated volumetric readouts, based on measurements of oxygen generated by a reaction between catalase and hydrogen peroxide, allow rapid ELISA quantitation of biomarkers and provide visualized bar charts on V- Chip without any assistance from instruments, data processing, or graphic plotting. The accuracy of the V-Chip will be validated in HCC cases and controls using standard ELISA assay. Based on epidemiological risk factors and biomarkers on V-chip, we will develop a risk prediction model to predict a person's probability of developing HCC in the next 5 and 10 years based on current risk profiles as well as risk reduction if the patient's modifiable risk factors change. This risks prediction V chip is particularly suitable for the application in resource-limited areas with higher HCC incidence. Patients can use V-Chip in home and personally monitor cirrhosis or HBV/HCV development by themselves, which may bring potential HCC patients to hospital and professionals earlier and thus greatly reduced the costs and risks of HCC.

Public Health Relevance

(PQA4) Point-of-Care Test of Exposure to Hepatocellular Carcinoma Risk by V-Chip Underlying mechanisms for synergisms of HCC risks may provide efficient and accurate HCC prevention strategies for high-risk individuals. People exposed to several risk factors simultaneously exhibit synergistically increased risks of HCC development. As hepatitis virus infections, especially hepatitis B virus (HBV), are endemic in the group of people in low income, developing an accurate, quantitative, and also low-cost technology for the rapid detection of biomarkers is required for HCC risk assessment. The development of the V-Chip holds the potential to meet the technological challenges listed in the PQA4 grant mechanism.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
1R01CA180083-01
Application #
8590772
Study Section
Special Emphasis Panel (ZCA1-SRLB-2 (M1))
Program Officer
Rinaudo, Jo Ann S
Project Start
2013-09-10
Project End
2017-06-30
Budget Start
2013-09-10
Budget End
2014-06-30
Support Year
1
Fiscal Year
2013
Total Cost
$534,498
Indirect Cost
$108,539
Name
Methodist Hospital Research Institute
Department
Type
DUNS #
185641052
City
Houston
State
TX
Country
United States
Zip Code
77030
Han, Xin; Liu, Zongbin; Zhao, Li et al. (2016) Microfluidic Cell Deformability Assay for Rapid and Efficient Kinase Screening with the CRISPR-Cas9 System. Angew Chem Int Ed Engl 55:8561-5
Liu, Zongbin; Han, Xin; Qin, Lidong (2016) Recent Progress of Microfluidics in Translational Applications. Adv Healthc Mater 5:871-88
Han, Xin; Liu, Zongbin; Jo, Myeong Chan et al. (2015) CRISPR-Cas9 delivery to hard-to-transfect cells via membrane deformation. Sci Adv 1:e1500454
Zhang, Yuanqing; Wen, Jianguo; Zhou, Ledu et al. (2015) Utilizing a high-throughput microfluidic platform to study hypoxia-driven mesenchymal-mode cell migration. Integr Biol (Camb) 7:672-80
Liu, Zongbin; Lee, Yeonju; Jang, Joon hee et al. (2015) Microfluidic cytometric analysis of cancer cell transportability and invasiveness. Sci Rep 5:14272
Li, Ying; Xuan, Jie; Xia, Tom et al. (2015) Competitive volumetric bar-chart chip with real-time internal control for point-of-care diagnostics. Anal Chem 87:3771-7
He, Fang; York, J Philippe; Burroughs, Sherilyn Gordon et al. (2015) Recruited metastasis suppressor NM23-H2 attenuates expression and activity of peroxisome proliferator-activated receptor δ (PPARδ) in human cholangiocarcinoma. Dig Liver Dis 47:62-7
Li, Ying; Xuan, Jie; Song, Yujun et al. (2015) A microfluidic platform with digital readout and ultra-low detection limit for quantitative point-of-care diagnostics. Lab Chip 15:3300-6
Jang, Joon Hee; Huang, Yu; Zheng, Peilin et al. (2015) Imaging of Cell-Cell Communication in a Vertical Orientation Reveals High-Resolution Structure of Immunological Synapse and Novel PD-1 Dynamics. J Immunol 195:1320-30
Su, Ping-Jung; Liu, Zongbin; Zhang, Kai et al. (2015) Retinal synaptic regeneration via microfluidic guiding channels. Sci Rep 5:13591

Showing the most recent 10 out of 19 publications