This proposal will examine the novel hypothesis the genesis and function of breast cancer stem cells (CSCs) are governed by a feed forward loop that sustains activation of the Hippo transducer TAZ. The nexus of this loop is a splice variant of the ?6?1 integrin, ?6B?1. Two variants of the ?6 subunit exist (?6A and ?6B) that differ onl in their cytoplasmic domains. Alternative splicing of a common mRNA generates these variants. ?6A?1 integrin signaling prevents TAZ activation.
The first aim will pursue the mechanism involved based on the findings that ?6A?1 interacts with the polarity/tumor suppressor protein Scribble and that Scribble associates with TAZ and prevents its nuclear localization. Thus, the first aim will test the hypothesis that the ?6A?1 integrin interacts with Scribble on the surfaceof mammary epithelial cells and contributes to its anchoring on the plasma membrane, an interaction mediated by the PDZ-binding domain present in the ?6A cytoplasmic domain. It is postulated that the ?6A integrin/Scribble complex sequesters TAZ preventing its nuclear localization and activation.
The second aim will assess the hypothesis that one mechanism by which TAZ promotes the genesis of CSCs is to repress the mRNA splicing factor ESRP1 resulting in the genesis of ?6B?1 and loss of ?6A?1, and the formation of a LM511 matrix. Moreover, it is postulated that TAZ regulates VEGF transcription and induces VEGF signaling, and that VEGF signaling enables the BMI1-mediated repression of ESRP1. TAZ also promotes the formation of a LM511 matrix that functions as the ligand for ?6B?1. Consequently, a feed forward loop is established involving TAZ and LM511/?6B?1 signaling that sustains CSCs. The third specific aim will determine how ?6A?1 and ?6B?1 signaling differ in their ability to actiate TAZ focusing on the role of Jun-terminal kinase (JNK). The specific hypothesis to be addressed is that ?6A?1 signaling promotes JNK activation and JNK-mediated phosphorylation of TAZ on a novel serine (S90), which contributes to TAZ inactivation. LM511/?6B?1 signaling, in contrast, prevents JNK activation and JNK-mediated TAZ phosphorylation enabling TAZ activation. This hypothesis infers that JNK inhibition has a causal role in TAZ activation and the acquisition of stem cell properties, which will be evaluated. This proposal is rich in innovation and significance and the results to be obtained will have a major impact on our understanding of the biology of breast CSCs and reveal new mechanisms for therapeutic intervention.

Public Health Relevance

This proposal seeks to understand mechanisms that contribute to the formation and growth of breast cancers. We propose to investigate how specific cell adhesion receptors work together with a fundamental pathway in development to regulate breast cancer initiation. The results to be obtained from these studies will have a major impact on our understanding of the biology of breast cancer and reveal new mechanisms for therapeutic intervention.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA203439-03
Application #
9383034
Study Section
Intercellular Interactions Study Section (ICI)
Program Officer
Espey, Michael G
Project Start
2015-12-28
Project End
2020-11-30
Budget Start
2017-12-01
Budget End
2018-11-30
Support Year
3
Fiscal Year
2018
Total Cost
Indirect Cost
Name
University of Massachusetts Medical School Worcester
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
603847393
City
Worcester
State
MA
Country
United States
Zip Code
01655
Brown, Caitlin W; Amante, John J; Goel, Hira Lal et al. (2017) The ?6?4 integrin promotes resistance to ferroptosis. J Cell Biol 216:4287-4297
Yoshii, Tatsuyuki; Geng, Yingying; Peyton, Shelly et al. (2016) Biochemical and biomechanical drivers of cancer cell metastasis, drug response and nanomedicine. Drug Discov Today 21:1489-1494