When addicts perceive conditioned stimuli (CSs) associated with drugs, they often find themselves engaging in drug-seeking behavior, even after a period of successful abstinence. Understanding how CSs promote drug- and reward-seeking behavior is thus of primary importance to understanding addiction and relapse. In this proposal, we focus on conditioned approach behavior: the conditioned locomotor response to the CS that often brings the subject closer to the predicted reward. These responses can be flexible in that the specific approach actions required to reach the target location can vary across encounters with the CS. We use a rat model of flexible conditioned approach to investigate the neural circuitry underlying this behavior. An important element of this circuitry is the nucleus accumbens (NAc), a brain region that contributes significantly to addictive behavior. Part of this contribution is likely due to the critical role it plays in flexible approach responses: these absolutely depend on the NAc and the dopamine projection it receives from the ventral tegmental area. However, it is not yet understood how NAc neurons dopamine-dependently facilitate flexible approach. One possibility ("Target Selection") is that some NAc neurons selects among different possible targets to approach based on the reward predicted by the CS and the target location. Other NAc neurons could serve a different function ("Approach Gating") of activating downstream circuits that determine the target and the approach actions, without themselves contributing to target selection. To understand conditioned approach behavior, it is essential to know whether NAc neurons serve only one of these functions (and if so, which one) or whether they participate in both of them. To test the Target Selection and Approach Gating hypotheses, we use multiple CSs, multiple targets to approach, and/or multiple outcomes to determine whether the CS-evoked firing responses of NAc neurons in behaving rats encode information about the approach target, the outcome predicted by the CS, or both. Next, we determine how the dopamine input to the NAc influences this encoding, using a powerful new technique developed in our lab for applying pharmacological compounds (dopamine antagonists and agonists) to the neurons we record from in behaving animals. In particular, we test the long-standing (but not yet directly tested) hypothesis that dopamine contributes the reward-predictive component of NAc neurons'encoding of CSs. Our technique allows us to establish different potential contributions of D1 and D2 classes of dopamine receptors to behaviorally-relevant NAc neuronal firing. Thus, these experiments will reveal specific circuit mechanisms whereby NAc neurons promote approach responses to CSs. By doing so, they will enhance our understanding of the control over addicts'drug-seeking behavior by drug-associated cues.

Public Health Relevance

One reason why drug addiction is a major public health problem is that addicts spend inordinate time and resources seeking and taking drugs. The research proposed here will help us to understand how a brain circuit that is critical for drug-seeking behavior controls how and when the subject pursues a reward. This information will lead to a mechanistic understanding of these neural circuits, and potentially to improved treatments for addiction.

Agency
National Institute of Health (NIH)
Institute
National Institute on Drug Abuse (NIDA)
Type
Research Project (R01)
Project #
5R01DA019473-09
Application #
8637028
Study Section
Neurobiology of Motivated Behavior Study Section (NMB)
Program Officer
Volman, Susan
Project Start
2005-04-01
Project End
2017-03-31
Budget Start
2014-04-01
Budget End
2015-03-31
Support Year
9
Fiscal Year
2014
Total Cost
$401,635
Indirect Cost
$161,135
Name
Albert Einstein College of Medicine
Department
Psychiatry
Type
Schools of Medicine
DUNS #
110521739
City
Bronx
State
NY
Country
United States
Zip Code
10461
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Morrison, Sara E; Nicola, Saleem M (2014) Neurons in the nucleus accumbens promote selection bias for nearer objects. J Neurosci 34:14147-62
McGinty, Vincent B; Lardeux, Sylvie; Taha, Sharif A et al. (2013) Invigoration of reward seeking by cue and proximity encoding in the nucleus accumbens. Neuron 78:910-22
Lardeux, Sylvie; Kim, James J; Nicola, Saleem M (2013) Intermittent access to sweet high-fat liquid induces increased palatability and motivation to consume in a rat model of binge consumption. Physiol Behav 114-115:21-31
du Hoffmann, Johann; Kim, James J; Nicola, Saleem M (2011) An inexpensive drivable cannulated microelectrode array for simultaneous unit recording and drug infusion in the same brain nucleus of behaving rats. J Neurophysiol 106:1054-64
Ambroggi, Frederic; Ghazizadeh, Ali; Nicola, Saleem M et al. (2011) Roles of nucleus accumbens core and shell in incentive-cue responding and behavioral inhibition. J Neurosci 31:6820-30
Nicola, Saleem M (2010) The flexible approach hypothesis: unification of effort and cue-responding hypotheses for the role of nucleus accumbens dopamine in the activation of reward-seeking behavior. J Neurosci 30:16585-600
Ishikawa, A; Ambroggi, F; Nicola, S M et al. (2008) Contributions of the amygdala and medial prefrontal cortex to incentive cue responding. Neuroscience 155:573-84
Ishikawa, Akinori; Ambroggi, Frederic; Nicola, Saleem M et al. (2008) Dorsomedial prefrontal cortex contribution to behavioral and nucleus accumbens neuronal responses to incentive cues. J Neurosci 28:5088-98
Ambroggi, Frederic; Ishikawa, Akinori; Fields, Howard L et al. (2008) Basolateral amygdala neurons facilitate reward-seeking behavior by exciting nucleus accumbens neurons. Neuron 59:648-61

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