The experiments proposed below are designed to examine development in the olfactory bulb and its principal synaptic targets, the anterior olfactory nucleus and primary olfactory cortex. The olfactory system has been chosen for several reasons. First, it is highly organized, facilitating quantitative examinations of early development and making subtle alterations relatively easy to discern. Many interesting forms of early plasticity have been demonstrated in the system, including inductive tissue interactions and substantial sensitivity to early stimutation. Indeed early unilateral odor deprivation has been repeatedly demonstrated to result in some of the largest experience-induced changes observed in the central nervous system. As a result the region affords considerable potential for examinations of the mechanisms by which environmental factors help to specify early growth. Two groups of experiments are proposed. The first continues our quantitative examination of early olfactory bulb development in the rat which emphasizes studies of how alterations in functional activity affect bulb growth. Specifically, we will examine: patterns of protein synthesis both in normally developing and deprived bulbs to examine the early cell biology of the olfactory system changes in the development of bulb glia populations. Another set of studies examines early bulb growth in the gray, short-tailed opossum Monodelphis domestics. Monodelphis pups are born in a very immature state, and are quite accessible to early experimental manipulation allowing in depth examinations of early tissue interactions. Specific experiments include: examinations of early functional deprivation to gauge how activity influences early growth. assessment of the effects of bulb removal on the development of higher order olfactory structures. In summary, the proposed research will explore early developmental processes such as the instructive interactions which occur between tissues and the role of afferent activity in growth and differentiation.

Agency
National Institute of Health (NIH)
Institute
National Institute on Deafness and Other Communication Disorders (NIDCD)
Type
Research Project (R01)
Project #
5R01DC000338-06
Application #
3216627
Study Section
Sensory Disorders and Language Study Section (CMS)
Project Start
1987-04-01
Project End
1995-12-31
Budget Start
1993-01-01
Budget End
1993-12-31
Support Year
6
Fiscal Year
1993
Total Cost
Indirect Cost
Name
University of Virginia
Department
Type
Schools of Arts and Sciences
DUNS #
001910777
City
Charlottesville
State
VA
Country
United States
Zip Code
22904
Collins, L N; Hill, D L; Brunjes, P C (2018) Myelination of the developing lateral olfactory tract and anterior commissure. J Comp Neurol 526:1843-1858
Kay, Rachel B; Brunjes, Peter C (2014) Diversity among principal and GABAergic neurons of the anterior olfactory nucleus. Front Cell Neurosci 8:111
Brunjes, Peter C; Collins, Lindsay N; Osterberg, Stephen K et al. (2014) The mouse olfactory peduncle. 3. Development of neurons, glia, and centrifugal afferents. Front Neuroanat 8:44
Brunjes, Peter C (2012) The mouse olfactory peduncle. 2.The anterior limb of the anterior commissure. Front Neuroanat 6:51
Brunjes, Peter C; Kay, Rachel B; Arrivillaga, J P (2011) The mouse olfactory peduncle. J Comp Neurol 519:2870-86
Kay, Rachel B; Meyer, Elizabeth Amory; Illig, Kurt R et al. (2011) Spatial distribution of neural activity in the anterior olfactory nucleus evoked by odor and electrical stimulation. J Comp Neurol 519:277-89
Brunjes, Peter C; Kenerson, Michael C (2010) The anterior olfactory nucleus: quantitative study of dendritic morphology. J Comp Neurol 518:1603-16
Illig, Kurt R; Eudy, Jennifer D (2009) Contralateral projections of the rat anterior olfactory nucleus. J Comp Neurol 512:115-23
Meyer, Elizabeth Amory; Illig, Kurt R; Brunjes, Peter C (2006) Differences in chemo- and cytoarchitectural features within pars principalis of the rat anterior olfactory nucleus suggest functional specialization. J Comp Neurol 498:786-95
Byrd, C A; Brunjes, P C (2001) Neurogenesis in the olfactory bulb of adult zebrafish. Neuroscience 105:793-801

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