Structural and functional features of the taste system change dramatically during development. While much has been learned by studying normal developmental processes, complementary experiments using experimental manipulations during critical phases of development have been of great value in learning how the taste system is organized.
The specific aims of this proposal use genetic knockout mouse models and early dietary manipulations with coordinated techniques of peripheral taste nerve recordings, central nervous system tract tracing, single cell fills of targeted brainstem neurons, in vivo central tast neuron neurophysiology, and in vitro patch-clamp experiments to study the role of activity-independent and activity-dependent factors on gustatory brainstem circuit development. Morphological studies examine 1) the terminal field organization of afferent nerves in the first synaptic relay in the brain, in the nucleus of the solitary tract (NTS), and 2) examine the dendritic characteristics of relay neurons in the NTS that project to the next central relay in mic, and putative inhibitory interneurons. Neurophysiological studies will examine 1) the influences of altered taste-elicited activity during development and early dietary manipulations on taste responses through in vivo single NTS cell recordings, and 2) influences on the intrinsic membrane properties and synaptic properties of NTS cells through in vitro patch- clamp slice recordings. The overall focus of this proposal is on how the central gustatory system is assembled and then refined by experience. Findings from these studies will provide new and important information about the development and plasticity of the peripheral and central gustatory system, and about the development of taste preferences and aversions. They will also be useful in determining the role that the maternal diet and taste-elicited activity have on organizing the developing sense of taste.

Public Health Relevance

One of the fundamental questions in neuroscience is how circuits in the brain are formed during development and how they are modified by experience. The proposed research program seeks to define fundamental aspects of how circuit development in the gustatory brainstem is influenced by neural activity and by non- activity dependent factors. Since taste plays a key role in food acceptance and rejection, our work may lead to new insights into how preferences and aversions are formed during development and how modifiable these circuits are through experience.

Agency
National Institute of Health (NIH)
Institute
National Institute on Deafness and Other Communication Disorders (NIDCD)
Type
Research Project (R01)
Project #
5R01DC000407-29
Application #
9207074
Study Section
Special Emphasis Panel (ZRG1-IFCN-B (03)M)
Program Officer
Sullivan, Susan L
Project Start
1986-08-01
Project End
2018-01-31
Budget Start
2017-02-01
Budget End
2018-01-31
Support Year
29
Fiscal Year
2017
Total Cost
$331,132
Indirect Cost
$121,555
Name
University of Virginia
Department
Psychology
Type
Schools of Arts and Sciences
DUNS #
065391526
City
Charlottesville
State
VA
Country
United States
Zip Code
22904
Collins, L N; Hill, D L; Brunjes, P C (2018) Myelination of the developing lateral olfactory tract and anterior commissure. J Comp Neurol 526:1843-1858
Sun, Chengsan; Krimm, Robin; Hill, David L (2018) Maintenance of Mouse Gustatory Terminal Field Organization is Dependent on BDNF at Adulthood. J Neurosci :
Sun, Chengsan; Hummler, Edith; Hill, David L (2017) Selective Deletion of Sodium Salt Taste during Development Leads to Expanded Terminal Fields of Gustatory Nerves in the Adult Mouse Nucleus of the Solitary Tract. J Neurosci 37:660-672
Skyberg, Rolf; Sun, Chengsan; Hill, David L (2017) Maintenance of Mouse Gustatory Terminal Field Organization Is Disrupted following Selective Removal of Peripheral Sodium Salt Taste Activity at Adulthood. J Neurosci 37:7619-7630
Dvoryanchikov, Gennady; Hernandez, Damian; Roebber, Jennifer K et al. (2017) Transcriptomes and neurotransmitter profiles of classes of gustatory and somatosensory neurons in the geniculate ganglion. Nat Commun 8:760
Meng, Lingbin; Huang, Tao; Sun, Chengsan et al. (2017) BDNF is required for taste axon regeneration following unilateral chorda tympani nerve section. Exp Neurol 293:27-42
Meng, Lingbin; Ohman-Gault, Lisa; Ma, Liqun et al. (2015) Taste Bud-Derived BDNF Is Required to Maintain Normal Amounts of Innervation to Adult Taste Buds. eNeuro 2:
Sun, Chengsan; Dayal, Arjun; Hill, David L (2015) Expanded terminal fields of gustatory nerves accompany embryonic BDNF overexpression in mouse oral epithelia. J Neurosci 35:409-21
Graham, Dustin M; Sun, Chengsan; Hill, David L (2014) Temporal signatures of taste quality driven by active sensing. J Neurosci 34:7398-411
Wang, Siting; Corson, James; Hill, David et al. (2012) Postnatal development of chorda tympani axons in the rat nucleus of the solitary tract. J Comp Neurol 520:3217-35

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