Abstract

The present proposal is part of an overall effort to understand basic and clinically relevant aspects of olfaction, in particular mechanisms of olfactory transduction in the rat. A multidisciplinary approach is proposed involving electrophysiological, biophysical, and molecular biological techniques. Experiments are proposed to understand how olfactory receptors stimulate the formation of two known olfactory second messengers, cAMP and InsP3, and how these intracellular signals regulate the membrane conductance of olfactory receptor neurons. Specifically, experiments are proposed to: (1) characterize the currents elicited by cAMP and Ins3-generating odors, (2) characterize the currents elicited by both these second messengers, (3) study the potential interaction among olfactory receptor neurons in situ in epithelial slices, (4) characterize olfactory receptor gene expression in single olfactory receptor neurons, and (5) analyze the odor specificity and sensitivity of any cloned olfactory receptor gene products in heterologous expression systems.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute on Deafness and Other Communication Disorders (NIDCD)
Type
Research Project (R01)
Project #
5R01DC000566-10
Application #
2443590
Study Section
Sensory Disorders and Language Study Section (CMS)
Project Start
1988-12-12
Project End
2001-06-30
Budget Start
1997-07-01
Budget End
1998-06-30
Support Year
10
Fiscal Year
1997
Total Cost
Indirect Cost

  Institution

Name
University of Colorado Denver
Department
Biology
Type
Schools of Medicine
DUNS #
065391526
City
Aurora
State
CO
Country
United States
Zip Code
80045

  Publications

Pouille, Frederic; Schoppa, Nathan E (2018) Cannabinoid Receptors Modulate Excitation of an Olfactory Bulb Local Circuit by Cortical Feedback. Front Cell Neurosci 12:47
Ozbay, Baris N; Futia, Gregory L; Ma, Ming et al. (2018) Three dimensional two-photon brain imaging in freely moving mice using a miniature fiber coupled microscope with active axial-scanning. Sci Rep 8:8108
Li, Anan; Guthman, Ethan M; Doucette, Wilder T et al. (2017) Behavioral Status Influences the Dependence of Odorant-Induced Change in Firing on Prestimulus Firing Rate. J Neurosci 37:1835-1852
Pouille, Frederic; McTavish, Thomas S; Hunter, Lawrence E et al. (2017) Intraglomerular gap junctions enhance interglomerular synchrony in a sparsely connected olfactory bulb network. J Physiol 595:5965-5986
Zhou, Yang; Wang, Xiaojie; Cao, Tiantian et al. (2017) Insulin Modulates Neural Activity of Pyramidal Neurons in the Anterior Piriform Cortex. Front Cell Neurosci 11:378
Castillo-Azofeifa, David; Losacco, Justin T; Salcedo, Ernesto et al. (2017) Sonic hedgehog from both nerves and epithelium is a key trophic factor for taste bud maintenance. Development 144:3054-3065
Bourne, Jennifer N; Schoppa, Nathan E (2017) Three-dimensional synaptic analyses of mitral cell and external tufted cell dendrites in rat olfactory bulb glomeruli. J Comp Neurol 525:592-609
Ozbay, Baris N; Losacco, Justin T; Cormack, Robert et al. (2015) Miniaturized fiber-coupled confocal fluorescence microscope with an electrowetting variable focus lens using no moving parts. Opt Lett 40:2553-6
Li, Anan; Gire, David H; Restrepo, Diego (2015) ? spike-field coherence in a population of olfactory bulb neurons differentiates between odors irrespective of associated outcome. J Neurosci 35:5808-22
ten Oever, Sanne; Schroeder, Charles E; Poeppel, David et al. (2014) Rhythmicity and cross-modal temporal cues facilitate detection. Neuropsychologia 63:43-50

Showing the most recent 10 out of 73 publications