Data now clearly establish that odors activate at least two second messenger-mediated transduction pathways in olfactory receptor cells, and that they do so in phylogenetically diverse animals. As yet, the functional basis for having more than one way to activate olfactory receptor cells is unknown, even though this knowledge is fundamental to understanding the sense of smell. For example, having multiple transduction pathways co-expressed in the same cells would provide direct evidence that olfactory integration begins at the level of receptor cell, while having them expressed in different cells would indicate functional subclasses of olfactory receptor cells. Either of these heretofore unknown elements of peripheral olfactory organization have direct implications as to how odors are coded. It is proposed to combine whole cell and single channel electrophysiological recording and biochemical analyses of second messenger production to characterize cAMP and IP3-regulated olfactory transduction. The study uses an animal model for which preliminary data establish that the two transduction pathways co-exist in the same receptor cells and regulate distinct, opposing conductances that act in concert to determine the electrophysiological response of the receptor cell to complex odors. The study provides the first opportunity to investigate these two transduction pathways in a functionally defined context. Understanding olfactory transduction is fundamental to understanding the role of the receptor cell in the sense of smell and, in turn, olfactory dysfunctions caused by environmental and pathogenic insult to the receptor organ.

Agency
National Institute of Health (NIH)
Institute
National Institute on Deafness and Other Communication Disorders (NIDCD)
Type
Research Project (R01)
Project #
5R01DC001655-04
Application #
2126656
Study Section
Sensory Disorders and Language Study Section (CMS)
Project Start
1992-08-01
Project End
1999-07-31
Budget Start
1995-08-01
Budget End
1996-07-31
Support Year
4
Fiscal Year
1995
Total Cost
Indirect Cost
Name
University of Florida
Department
Type
Organized Research Units
DUNS #
073130411
City
Gainesville
State
FL
Country
United States
Zip Code
32611
Bobkov, Y; Corey, E; Ache, B (2014) An inhibitor of Na(+)/Ca(2+) exchange blocks activation of insect olfactory receptors. Biochem Biophys Res Commun 450:1104-9
Ukhanov, K; Bobkov, Y; Corey, E A et al. (2014) Ligand-selective activation of heterologously-expressed mammalian olfactory receptor. Cell Calcium 56:245-56
Park, Il Memming; Bobkov, Yuriy V; Ache, Barry W et al. (2014) Intermittency coding in the primary olfactory system: a neural substrate for olfactory scene analysis. J Neurosci 34:941-52
Pask, Gregory M; Bobkov, Yuriy V; Corey, Elizabeth A et al. (2013) Blockade of insect odorant receptor currents by amiloride derivatives. Chem Senses 38:221-9
Ukhanov, Kirill; Corey, Elizabeth A; Ache, Barry W (2013) Phosphoinositide 3-kinase dependent inhibition as a broad basis for opponent coding in Mammalian olfactory receptor neurons. PLoS One 8:e61553
Corey, Elizabeth A; Bobkov, Yuriy; Ukhanov, Kirill et al. (2013) Ionotropic crustacean olfactory receptors. PLoS One 8:e60551
Bobkov, Yuriy; Park, Il; Park, Ill et al. (2012) Cellular basis for response diversity in the olfactory periphery. PLoS One 7:e34843
Bobkov, Y V; Corey, E A; Ache, B W (2011) The pore properties of human nociceptor channel TRPA1 evaluated in single channel recordings. Biochim Biophys Acta 1808:1120-8
Ukhanov, K; Bobkov, Y; Ache, B W (2011) Imaging ensemble activity in arthropod olfactory receptor neurons in situ. Cell Calcium 49:100-7
Ukhanov, Kirill; Brunert, Daniela; Corey, Elizabeth A et al. (2011) Phosphoinositide 3-kinase-dependent antagonism in mammalian olfactory receptor neurons. J Neurosci 31:273-80

Showing the most recent 10 out of 42 publications