Mammals explore the chemical world with two distinct olfactory modalities, known as the main and accessory olfactory systems. The accessory olfactory system (AOS) is at least partially specialized to detect cues, often called pheromones, which regulate social behavior among members of the same species. While many insect pheromones have been identified, the understanding of the nature of these cues in mice and other mammals is still rudimentary. One powerful strategy for identifying mammalian chemical social cues is to record from the sensory neurons of the AOS, and use their spiking responses to guide the purification of active compounds from natural sources such as urine. This approach recently led to our identification of sulfated steroids as the predominant source of activity in female mouse urine. Because steroids control mammalian physiology, their metabolites represent an attractive class of social cue, revealing much about the physiological state of other members of the group. This application proposes experiments to learn more about mammalian pheromones and the neurobiology of their detection. (1) Preliminary evidence suggests many uncharacterized steroid derivatives present in urine activate sensory neurons;chief among these are compounds that appear to be derivatives of estrogen, potentially signaling information about reproductive state. By combining biochemical purification, electrophysiology, and chemical analysis, some of these other compounds will be identified. (2) Individual sulfated steroids in urine vary in their level depending on an animal's physiological state. By recording the responses of the sensory neurons to a concentration series of different sulfated steroids, the mechanisms for encoding identity and concentration will be elucidated. These experiments will also reveal whether responsive neurons fall into affinity distinct classes. (3) The detailed understanding of steroid biology facilitates the making of hypotheses about the likely behavioral role of particular sulfated steroid pheromones. Behavioral experiments probing alarm/stress, dominance, and attractiveness will reveal the precise function of individual compounds in organizing social behavior.

Public Health Relevance

These experiments will reveal key properties of a major modality of mammalian communication. Because of its tractability, exploring this modality is sure to reveal general principles of sensory and circuit function with relevance to the organization of the brain in both health and disease.

Agency
National Institute of Health (NIH)
Institute
National Institute on Deafness and Other Communication Disorders (NIDCD)
Type
Research Project (R01)
Project #
5R01DC005964-10
Application #
8472470
Study Section
Somatosensory and Chemosensory Systems Study Section (SCS)
Program Officer
Sullivan, Susan L
Project Start
2003-04-01
Project End
2014-06-30
Budget Start
2013-07-01
Budget End
2014-06-30
Support Year
10
Fiscal Year
2013
Total Cost
$294,061
Indirect Cost
$100,600
Name
Washington University
Department
Neurosciences
Type
Schools of Medicine
DUNS #
068552207
City
Saint Louis
State
MO
Country
United States
Zip Code
63130
Yan, Yuetian; Ubukata, Masaaki; Cody, Robert B et al. (2014) High-energy collision-induced dissociation by MALDI TOF/TOF causes charge-remote fragmentation of steroid sulfates. J Am Soc Mass Spectrom 25:1404-11
Yan, Yuetian; Rempel, Don L; Holy, Timothy E et al. (2014) Mass spectrometry combinations for structural characterization of sulfated-steroid metabolites. J Am Soc Mass Spectrom 25:869-79
Miller, Jae-Eun Kang; Granados-Fuentes, Daniel; Wang, Thomas et al. (2014) Vasoactive intestinal polypeptide mediates circadian rhythms in mammalian olfactory bulb and olfaction. J Neurosci 34:6040-6
Xu, Pei Sabrina; Holy, Timothy E (2013) Whole-mount imaging of responses in mouse vomeronasal neurons. Methods Mol Biol 1068:201-10
Tolokh, Illya I; Fu, Xiaoyan; Holy, Timothy E (2013) Reliable sex and strain discrimination in the mouse vomeronasal organ and accessory olfactory bulb. J Neurosci 33:13903-13
Arnson, Hannah A; Holy, Timothy E (2013) Robust encoding of stimulus identity and concentration in the accessory olfactory system. J Neurosci 33:13388-97
Turaga, Diwakar; Holy, Timothy E (2012) Organization of vomeronasal sensory coding revealed by fast volumetric calcium imaging. J Neurosci 32:1612-21
Arnson, Hannah A; Holy, Timothy E (2011) Chemosensory burst coding by mouse vomeronasal sensory neurons. J Neurophysiol 106:409-20
Meeks, Julian P; Arnson, Hannah A; Holy, Timothy E (2010) Representation and transformation of sensory information in the mouse accessory olfactory system. Nat Neurosci 13:723-30
Arnson, Hannah A; Fu, Xiaoyan; Holy, Timothy E (2010) Multielectrode array recordings of the vomeronasal epithelium. J Vis Exp :

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