Skeletal muscles, including those of the tongue and larynx, are capable of considerable plasticity based on changing demands and levels of activity. The biological mechanisms that influence lingual and laryngeal muscular plasticity are not well defined or well studied. This limits our ability to design and carry-out effective rehabilitation fr voice and swallowing disorders. Aging allows examination of naturally occurring muscular plasticity and how to intervene towards prevention or reversal of suboptimal sensorimotor changes throughout the adult lifespan. Senescent individuals exhibit a pattern of structural and functional adaptations within muscles and the vascular system that may contribute to fatigue and weakness, such as alterations in microvascular geometry and blood flow, accelerated programmed cell death (apoptosis), and impaired regenerative capacity via muscle stem cells (satellite cells). We will use and extend a tongue exercise-water swallow paradigm developed in our lab to examine lingual and laryngeal musculoplasticity as a function of aging and exercise. Because muscles involved in the control of vocalization and swallowing share many neuromuscular control properties, tongue exercise paired with a water swallow may theoretically benefit both lingual and laryngeal functions. However, the manner in which this type of cranial cross-system exercise is manifested at the muscle level is unclear, and it is not known how musculoplastic adaptations affect vocalization and swallowing behaviors. Our hypothesis is that lingual and laryngeal muscle structure and physiology are altered throughout the adult lifespan and that age-related cranial musculoplasticity contributes to deficits observed in vocalization and deglutition. We hypothesize that mechanisms are alterations in lingual and laryngeal muscle regenerative capacity, programmed cell death, microvascular geometry and blood flow, and that these variables can be positively influenced by tongue exercise. We will test these hypotheses in rats of different ages that have undergone tongue exercise versus control conditions. We have 3 specific aims. In young adult, middle-aged and old rats, we will: (1) Quantify mechanisms of lingual and laryngeal muscle plasticity using an aging rat tongue exercise model;(2) Quantify changes in lingual and laryngeal muscle microvascular geometry and red blood cell (RBC) velocity with aging and exercise;(3) Determine how aging and tongue exercise affect vocalization and deglutition behavior. This work is innovative and significant because the mechanisms by which exercise impacts protective effects in the lingual and laryngeal sensorimotor systems are largely unexplored. Our neuromuscular model is the first to evaluate the effects of a behavioral exercise program on lingual and laryngeal muscle plasticity and on causal pathways. Further, this work is highly significant in providing a basis for understanding mechanisms underlying the potential benefits of exercise as a therapeutic intervention for muscular adaptation in cranial muscles. Translation of findings will assist with increasing effectiveness and efficiency of exercise-based voice and swallow treatments.

Public Health Relevance

Muscles can undergo substantial deterioration in structure and function when they are compromised by aging and disease and can make positive changes with rehabilitation. However, biological factors underlying these processes have been largely undefined in muscles of the tongue and larynx. Our research uses a rat model to determine how exercise tasks may change tongue and larynx muscles. This work is highly relevant to human voice and swallowing rehabilitation because we need specific scientific data to help make behavioral therapies more effective and efficient.

Agency
National Institute of Health (NIH)
Institute
National Institute on Deafness and Other Communication Disorders (NIDCD)
Type
Research Project (R01)
Project #
2R01DC008149-06A1
Application #
8583043
Study Section
Motor Function, Speech and Rehabilitation Study Section (MFSR)
Program Officer
Shekim, Lana O
Project Start
2006-04-01
Project End
2018-06-30
Budget Start
2013-07-01
Budget End
2014-06-30
Support Year
6
Fiscal Year
2013
Total Cost
$536,147
Indirect Cost
$179,903
Name
University of Wisconsin Madison
Department
Surgery
Type
Schools of Medicine
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715
Connor, Nadine P; Russell, John A; Jackson, Michelle A et al. (2013) Tongue muscle plasticity following hypoglossal nerve stimulation in aged rats. Muscle Nerve 47:230-40
Johnson, Aaron M; Ciucci, Michelle R; Connor, Nadine P (2013) Vocal training mitigates age-related changes within the vocal mechanism in old rats. J Gerontol A Biol Sci Med Sci 68:1458-68
Kletzien, Heidi; Russell, John A; Leverson, Glen E et al. (2013) Differential effects of targeted tongue exercise and treadmill running on aging tongue muscle structure and contractile properties. J Appl Physiol 114:472-81
Ciucci, Michelle R; Schaser, Allison J; Russell, John A (2013) Exercise-induced rescue of tongue function without striatal dopamine sparing in a rat neurotoxin model of Parkinson disease. Behav Brain Res 252:239-45
Behan, Mary; Moeser, Adam E; Thomas, Cathy F et al. (2012) The effect of tongue exercise on serotonergic input to the hypoglossal nucleus in young and old rats. J Speech Lang Hear Res 55:919-29
Schaser, Allison J; Stang, Kyle; Connor, Nadine P et al. (2012) The effect of age and tongue exercise on BDNF and TrkB in the hypoglossal nucleus of rats. Behav Brain Res 226:235-41
Ciucci, Michelle R; Russell, John A; Schaser, Allison J et al. (2011) Tongue force and timing deficits in a rat model of Parkinson disease. Behav Brain Res 222:315-20
Johnson, Aaron M; Connor, Nadine P (2011) Effects of electrical stimulation on neuromuscular junction morphology in the aging rat tongue. Muscle Nerve 43:203-11
Schaser, Allison J; Wang, Hao; Volz, Lana M et al. (2011) Biochemistry of the anterior, medial, and posterior genioglossus in the aged rat. Dysphagia 26:256-63
Ciucci, Michelle R; Vinney, Lisa; Wahoske, Emerald J et al. (2010) A translational approach to vocalization deficits and neural recovery after behavioral treatment in Parkinson disease. J Commun Disord 43:319-26

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