Understanding the structure of cortical synaptic circuits is key to comprehending information representation and processing in the auditory cortex. However, due to technical limitations, the general structure of cortical synaptic circuits, and how this structure determines cortical function, remains largely unknown. As a first step to addressing this issue, in this project, we will investigate the patterns of excitatory and inhibitory synaptic inputs underlying the functional responses of individual cortical neurons and reveal the synaptic mechanisms determining or shaping these response properties. In the auditory cortex, patterns of synaptic inputs can be largely reflected by their frequency-intensity tonal receptive fields (TRFs). These patterns represent basic structural properties of synaptic input circuitry underlying the functioning of individual cortical neurons. Using an in vivo whole-cell recording technique, we will determine the """"""""spectrotemporal"""""""" pattern of synaptic inputs for both excitatory and inhibitory neurons in the input layers of the adult rat auditory cortex. We will dissect the thalamocortical components of excitatory inputs by pharmacologically silencing the cortex. The cell type of recorded neurons will be determined by their spiking and morphological properties. We will determine excitatory and inhibitory synaptic mechanisms for the frequency/ intensity tuning of cortical pyramidal neurons by revealing the patterns of excitatory and inhibitory synaptic inputs with in vivo whole-cell voltage-clamp recording techniques. We will explicate the contribution of thalamocortical excitaotry inputs to the response properties of cortical neurons by developing a novel pharmacological approach to effectively and specifically silence the cortex. Finally, by distinguishing cortical inhibitory neurons according to histology and physiology, we will determine response properties of cortical GABAergic interneurons, and their underlying synaptic mechanisms.

Agency
National Institute of Health (NIH)
Institute
National Institute on Deafness and Other Communication Disorders (NIDCD)
Type
Research Project (R01)
Project #
5R01DC008983-06
Application #
8299098
Study Section
Auditory System Study Section (AUD)
Program Officer
Platt, Christopher
Project Start
2007-08-01
Project End
2013-12-31
Budget Start
2012-08-01
Budget End
2013-07-31
Support Year
6
Fiscal Year
2012
Total Cost
$308,350
Indirect Cost
$119,178
Name
University of Southern California
Department
Physiology
Type
Schools of Medicine
DUNS #
072933393
City
Los Angeles
State
CA
Country
United States
Zip Code
90089
Ji, Xu-Ying; Zingg, Brian; Mesik, Lukas et al. (2016) Thalamocortical Innervation Pattern in Mouse Auditory and Visual Cortex: Laminar and Cell-Type Specificity. Cereb Cortex 26:2612-25
Kim, Young J; Ibrahim, Leena A; Wang, Sheng-Zhi et al. (2016) EphA7 regulates spiral ganglion innervation of cochlear hair cells. Dev Neurobiol 76:452-69
Ibrahim, Leena A; Mesik, Lukas; Ji, Xu-Ying et al. (2016) Cross-Modality Sharpening of Visual Cortical Processing through Layer-1-Mediated Inhibition and Disinhibition. Neuron 89:1031-45
Kim, Young J; Wang, Sheng-zhi; Tymanskyj, Stephen et al. (2016) Dcc Mediates Functional Assembly of Peripheral Auditory Circuits. Sci Rep 6:23799
Tao, Can; Zhang, Guangwei; Zhou, Chang et al. (2016) Synaptic Basis for the Generation of Response Variation in Auditory Cortex. Sci Rep 6:31024
Li, Ling-Yun; Xiong, Xiaorui R; Ibrahim, Leena A et al. (2015) Differential Receptive Field Properties of Parvalbumin and Somatostatin Inhibitory Neurons in Mouse Auditory Cortex. Cereb Cortex 25:1782-91
Albers, Mark W; Gilmore, Grover C; Kaye, Jeffrey et al. (2015) At the interface of sensory and motor dysfunctions and Alzheimer's disease. Alzheimers Dement 11:70-98
Liang, Feixue; Xiong, Xiaorui R; Zingg, Brian et al. (2015) Sensory Cortical Control of a Visually Induced Arrest Behavior via Corticotectal Projections. Neuron 86:755-67
Zhou, Mu; Li, Ya-Tang; Yuan, Wei et al. (2015) Synaptic mechanisms for generating temporal diversity of auditory representation in the dorsal cochlear nucleus. J Neurophysiol 113:1358-68
Mesik, Lukas; Ma, Wen-pei; Li, Ling-yun et al. (2015) Functional response properties of VIP-expressing inhibitory neurons in mouse visual and auditory cortex. Front Neural Circuits 9:22

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