The brain has the remarkable capability to change in response to experience. While the entire nervous system is highly labile during development, the cerebral cortex remains plastic throughout life. This plasticity is essential for learning and memory, and is an important feature of the auditory cortex, especially for learning the significance of sensory signals such as speech, for the use of devices such as cochlear implants, and for recovery after short-term deafness. These changes are thought to occur primarily at synapses, basic units of information processing and plasticity. Long-term synaptic plasticity requires sensory experience and activation of neuromodulatory systems which convey behavioral context to local cortical circuits. However, little is known about the interactions between synaptic inputs and release of neuromodulators in vivo, making it challenging to relate perceptual learning to plasticity in the auditory cortex or other brain areas. Recently we have developed an approach to measuring the dynamics of synaptic modifications for hours, to more closely examine the links between auditory cortical plasticity and auditory perceptual learning. These experiments now allow the construction of a new framework for understanding general mechanisms of modulation and plasticity in a behavioral context. Specifically, we will study the physiological role of cortical and thalamic plasticity for enhancing auditory perception when sounds are paired with the powerful neuromodulator norepinephrine. Norepinephrine is important for selective attention, general arousal, and learning, is a major factor in stress, and s released by the locus coeruleus, a small and relatively homogeneous brainstem nucleus amenable to direct electrophysiological recordings. This proposal describes a series of electrophysiological and behavioral experiments that will examine the effects of locus coeruleus stimulation and norepinephrine release on the auditory cortex of adult rats. First, locus coeruleus stimulation will be paired with auditory stimuli in anesthetized animals for detailed intracellular recordings and mechanistic studies. Next, locus coeruleus pairing will be performed in awake animals to document the effects of neuromodulation and cortical plasticity on two forms of auditory behavior involving positive reward-based or negative stressful reinforcement. Finally, as preliminary data suggest that this form of plasticity is unusually long-lived and resistant to extinction, recordings will be made from locus coeruleus neurons to ask if this neuromodulatory center becomes sensitized to auditory stimulation to 'lock-in'changes of cortical circuitry via more continuous modulation. In summary, here we will use in vivo electrophysiological methods to ask how noradrenergic modulation, paired with acoustic input, leads to short- and long-term modifications of auditory thalamocortical circuitry and neuromodulatory release itself, to persistently improve perceptual abilities in behaving animals.

Public Health Relevance

Neuroplasticity - the ability of the brain to change in response to experience - is an essential feature of the auditory cortex, especially for speech and language learning as well as the successful use of devices such as cochlear implants. However, it is unclear how motivational state, behavioral training, and device usage drive plasticity within the central auditory system. The experiments to be performed in this proposal provide essential data on basic auditory neuroplastic mechanisms, required for improvement of prosthetic design and therapeutic strategies for treatment of deafness and language disorders.

Agency
National Institute of Health (NIH)
Institute
National Institute on Deafness and Other Communication Disorders (NIDCD)
Type
Research Project (R01)
Project #
1R01DC012557-01A1
Application #
8456624
Study Section
Auditory System Study Section (AUD)
Program Officer
Platt, Christopher
Project Start
2012-12-02
Project End
2017-11-30
Budget Start
2012-12-02
Budget End
2013-11-30
Support Year
1
Fiscal Year
2013
Total Cost
$412,809
Indirect Cost
$162,809
Name
New York University
Department
Otolaryngology
Type
Schools of Medicine
DUNS #
121911077
City
New York
State
NY
Country
United States
Zip Code
10016
Cohen, Samuel M; Ma, Huan; Kuchibhotla, Kishore V et al. (2016) Excitation-Transcription Coupling in Parvalbumin-Positive Interneurons Employs a Novel CaM Kinase-Dependent Pathway Distinct from Excitatory Neurons. Neuron 90:292-307
King, Julia; Shehu, Ina; Roland Jr, J Thomas et al. (2016) A physiological and behavioral system for hearing restoration with cochlear implants. J Neurophysiol 116:844-58
Insanally, Michele; Trumpis, Michael; Wang, Charles et al. (2016) A low-cost, multiplexed μECoG system for high-density recordings in freely moving rodents. J Neural Eng 13:026030
Mitre, Mariela; Marlin, Bianca J; Schiavo, Jennifer K et al. (2016) A Distributed Network for Social Cognition Enriched for Oxytocin Receptors. J Neurosci 36:2517-35
Martins, Ana Raquel O; Froemke, Robert C (2015) Coordinated forms of noradrenergic plasticity in the locus coeruleus and primary auditory cortex. Nat Neurosci 18:1483-92
Froemke, Robert C; Schreiner, Christoph E (2015) Synaptic plasticity as a cortical coding scheme. Curr Opin Neurobiol 35:185-99
Costa, Rui Ponte; Froemke, Robert C; Sjöström, P Jesper et al. (2015) Correction: Unified pre- and postsynaptic long-term plasticity enables reliable and flexible learning. Elife 4:e11988
Marlin, Bianca J; Mitre, Mariela; D'amour, James A et al. (2015) Oxytocin enables maternal behaviour by balancing cortical inhibition. Nature 520:499-504
Froemke, Robert C (2015) Plasticity of cortical excitatory-inhibitory balance. Annu Rev Neurosci 38:195-219
King, J; Insanally, M; Jin, M et al. (2015) Rodent auditory perception: Critical band limitations and plasticity. Neuroscience 296:55-65

Showing the most recent 10 out of 16 publications