The sense of smell is an early warning system for the detection of environmental hazards such as spoiled food, natural gas leaks, smoke, or airborne pollution. Smells also play an important role in quality of life, imparting the flavor of foods and beverages. The long- term objective of this proposal is to understand the nature of odor coding at the receptor level. Currently, little is known about how changes in the receptor activation pattern alter olfactory perception. In the research proposed here, we use next-generation sequencing techniques, heterologous expression systems, and human psychophysics to understand the links between receptor genotype, receptor function and olfactory perception. Learning the rules for transforming receptor activity into perception will advance our understanding of odor coding.

Public Health Relevance

The inability to smell environmental hazards or foods can affect both health and quality of life. Establishing a link between receptors and ligands will allo for the development of receptor agonists and antagonists to be used in direct therapeutic interventions.

Agency
National Institute of Health (NIH)
Institute
National Institute on Deafness and Other Communication Disorders (NIDCD)
Type
Research Project (R01)
Project #
1R01DC013339-01
Application #
8562603
Study Section
Somatosensory and Chemosensory Systems Study Section (SCS)
Program Officer
Sullivan, Susan L
Project Start
2013-07-12
Project End
2018-06-30
Budget Start
2013-07-12
Budget End
2014-06-30
Support Year
1
Fiscal Year
2013
Total Cost
$380,485
Indirect Cost
$107,550
Name
Monell Chemical Senses Center
Department
Type
DUNS #
088812565
City
Philadelphia
State
PA
Country
United States
Zip Code
19104
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Trimmer, Casey; Mainland, Joel D (2017) Simplifying the Odor Landscape. Chem Senses 42:177-179
Boesveldt, Sanne; Postma, Elbrich M; Boak, Duncan et al. (2017) Anosmia-A Clinical Review. Chem Senses 42:513-523
Ibarra-Soria, Ximena; Nakahara, Thiago S; Lilue, Jingtao et al. (2017) Variation in olfactory neuron repertoires is genetically controlled and environmentally modulated. Elife 6:
Mainland, Joel D; Li, Yun R; Zhou, Ting et al. (2015) Human olfactory receptor responses to odorants. Sci Data 2:150002
Trimmer, Casey; Snyder, Lindsey L; Mainland, Joel D (2014) High-throughput analysis of mammalian olfactory receptors: measurement of receptor activation via luciferase activity. J Vis Exp :
Mainland, Joel D; Keller, Andreas; Li, Yun R et al. (2014) The missense of smell: functional variability in the human odorant receptor repertoire. Nat Neurosci 17:114-20
Mainland, Joel D; Lundström, Johan N; Reisert, Johannes et al. (2014) From molecule to mind: an integrative perspective on odor intensity. Trends Neurosci 37:443-54
Mainland, Joel D; Willer, Jason R; Matsunami, Hiroaki et al. (2013) Next-generation sequencing of the human olfactory receptors. Methods Mol Biol 1003:133-47