The unique structural features of teeth and bones appear to have important roles in their resistance to disease. We will evaluate the role of nano-structural characteristics of bone and enamel in providing the nanosize-based resistance to dissolution recently documented for calcium phosphate nanocrystals. Initial contacts between an organic matrix and mineral nuclei are presumed to be crucial for the highly structured mineralization of teeth and bones by being imprinted on the mineral-matrix composite during initial formation events. Moreover, the presence of soluble proteins in bone and tooth mineral and observations of mineral growth in their presence suggest they act as control agents over growth rates and morphologies. Although many in vitro studies have looked at mineralization of collagen and dentin, they have not emphasized the earliest events during which mineral phase, position, morphology and orientation are determined. In particular, almost nothing is known about the pathway of crystallization from solvated ions to final apatitic mineral phase, nor has the potential existence of an amorphous precursor phase been explored. In this project, we will use constant composition, in situ AFM, and molecular modeling to determine the kinetics and energetics of apatite formation on dentin and collagen surfaces at realistic driving forces, determine the evolution of phases, identify the sites of nucleation, and define the structural relationships and stereochemical interactions that govern mineral formation on these matrices. Solid phases will be investigated by high resolution scanning and transmission electron microscopy, EDX, small/wide-angle X-ray scattering, ESCA, SIMS, differential scanning calorimetry, and zeta potential. The effect of important soluble proteins including amelogenin, osteopontin and their potentially functional peptide domains will be investigated. ? ? ?

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Research Project (R01)
Project #
2R01DE003223-36
Application #
7383345
Study Section
Skeletal Biology Development and Disease Study Section (SBDD)
Program Officer
Shum, Lillian
Project Start
1979-02-01
Project End
2013-04-30
Budget Start
2008-05-15
Budget End
2009-04-30
Support Year
36
Fiscal Year
2008
Total Cost
$429,198
Indirect Cost
Name
State University of New York at Buffalo
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
038633251
City
Buffalo
State
NY
Country
United States
Zip Code
14260
Tao, Jinhui; Battle, Keith C; Pan, Haihua et al. (2015) Energetic basis for the molecular-scale organization of bone. Proc Natl Acad Sci U S A 112:326-31
Borah, Ballav M; Halter, Timothy J; Xie, Baoquan et al. (2014) Kinetics of canine dental calculus crystallization: an in vitro study on the influence of inorganic components of canine saliva. J Colloid Interface Sci 425:20-6
Friddle, Raymond W; Battle, Keith; Trubetskoy, Vasily et al. (2011) Single-molecule determination of the face-specific adsorption of Amelogenin's C-terminus on hydroxyapatite. Angew Chem Int Ed Engl 50:7541-5
Giocondi, Jennifer L; El-Dasher, Bassem S; Nancollas, George H et al. (2010) Molecular mechanisms of crystallization impacting calcium phosphate cements. Philos Trans A Math Phys Eng Sci 368:1937-61
Xie, Baoquan; Nancollas, George H (2010) How to control the size and morphology of apatite nanocrystals in bone. Proc Natl Acad Sci U S A 107:22369-70
Yang, Xiudong; Wang, Lijun; Qin, Yueling et al. (2010) How amelogenin orchestrates the organization of hierarchical elongated microstructures of apatite. J Phys Chem B 114:2293-300
Roelofs, Anke J; Coxon, Fraser P; Ebetino, Frank H et al. (2010) Fluorescent risedronate analogues reveal bisphosphonate uptake by bone marrow monocytes and localization around osteocytes in vivo. J Bone Miner Res 25:606-16
Wang, Lijun; Nancollas, George H (2010) Dynamics of Biomineralization and Biodemineralization. Met Ions Life Sci 4:413-456
Nancollas, George H; Henneman, Zachary J (2010) Calcium oxalate: calcium phosphate transformations. Urol Res 38:277-80
Wang, Lijun; Nancollas, George H (2009) Pathways to biomineralization and biodemineralization of calcium phosphates: the thermodynamic and kinetic controls. Dalton Trans :2665-72

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