The unique structural features of teeth and bones appear to have important roles in their resistance to disease. We will evaluate the role of nano-structural characteristics of bone and enamel in providing the nanosize-based resistance to dissolution recently documented for calcium phosphate nanocrystals. Initial contacts between an organic matrix and mineral nuclei are presumed to be crucial for the highly structured mineralization of teeth and bones by being imprinted on the mineral-matrix composite during initial formation events. Moreover, the presence of soluble proteins in bone and tooth mineral and observations of mineral growth in their presence suggest they act as control agents over growth rates and morphologies. Although many in vitro studies have looked at mineralization of collagen and dentin, they have not emphasized the earliest events during which mineral phase, position, morphology and orientation are determined. In particular, almost nothing is known about the pathway of crystallization from solvated ions to final apatitic mineral phase, nor has the potential existence of an amorphous precursor phase been explored. In this project, we will use constant composition, in situ AFM, and molecular modeling to determine the kinetics and energetics of apatite formation on dentin and collagen surfaces at realistic driving forces, determine the evolution of phases, identify the sites of nucleation, and define the structural relationships and stereochemical interactions that govern mineral formation on these matrices. Solid phases will be investigated by high resolution scanning and transmission electron microscopy, EDX, small/wide-angle X-ray scattering, ESCA, SIMS, differential scanning calorimetry, and zeta potential. The effect of important soluble proteins including amelogenin, osteopontin and their potentially functional peptide domains will be investigated.

National Institute of Health (NIH)
National Institute of Dental & Craniofacial Research (NIDCR)
Research Project (R01)
Project #
Application #
Study Section
Skeletal Biology Development and Disease Study Section (SBDD)
Program Officer
Wan, Jason
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
State University of New York at Buffalo
Schools of Arts and Sciences
United States
Zip Code
Tao, Jinhui; Battle, Keith C; Pan, Haihua et al. (2015) Energetic basis for the molecular-scale organization of bone. Proc Natl Acad Sci U S A 112:326-31
Borah, Ballav M; Halter, Timothy J; Xie, Baoquan et al. (2014) Kinetics of canine dental calculus crystallization: an in vitro study on the influence of inorganic components of canine saliva. J Colloid Interface Sci 425:20-6
Friddle, Raymond W; Battle, Keith; Trubetskoy, Vasily et al. (2011) Single-molecule determination of the face-specific adsorption of Amelogenin's C-terminus on hydroxyapatite. Angew Chem Int Ed Engl 50:7541-5
Giocondi, Jennifer L; El-Dasher, Bassem S; Nancollas, George H et al. (2010) Molecular mechanisms of crystallization impacting calcium phosphate cements. Philos Trans A Math Phys Eng Sci 368:1937-61
Xie, Baoquan; Nancollas, George H (2010) How to control the size and morphology of apatite nanocrystals in bone. Proc Natl Acad Sci U S A 107:22369-70
Yang, Xiudong; Wang, Lijun; Qin, Yueling et al. (2010) How amelogenin orchestrates the organization of hierarchical elongated microstructures of apatite. J Phys Chem B 114:2293-300
Roelofs, Anke J; Coxon, Fraser P; Ebetino, Frank H et al. (2010) Fluorescent risedronate analogues reveal bisphosphonate uptake by bone marrow monocytes and localization around osteocytes in vivo. J Bone Miner Res 25:606-16
Wang, Lijun; Nancollas, George H (2010) Dynamics of Biomineralization and Biodemineralization. Met Ions Life Sci 4:413-456
Nancollas, George H; Henneman, Zachary J (2010) Calcium oxalate: calcium phosphate transformations. Urol Res 38:277-80
Weaver, Matthew L; Qiu, S Roger; Hoyer, John R et al. (2009) Surface aggregation of urinary proteins and aspartic Acid-rich peptides on the faces of calcium oxalate monohydrate investigated by in situ force microscopy. Calcif Tissue Int 84:462-73

Showing the most recent 10 out of 53 publications