A key element of this proposal is to develop an in situ system for an in depth analysis of the stimulus-secretion-synthesis cycle of a mucin from the submandibular glands of mice. The information gathered will be applied toward understanding mechanisms for control of synthesis and processing of salivary mucin. The stimulus-secretion-synthesis system will be standardized so that what is learned may serve as reference for future studies of salivary gland dysfunction such as xerostomia, sialorrhea and chronic enlargement. The amounts, rates of synthesis and rates of degradation of the mucin will be quantitated at each phase of the cycle by utilizing radioimmunoassay and immunoprecipitation of mucin polysomes. In addition, a protocol is described that will provide accurate timing of the glycosylation events. Possible control interactions between translation and oligosaccharide assembly will be investigated with the help of tunicamycin. The effects of various doses of different secretagogues on the stimulus-secretion-synthesis cycle will be compared. A procedure which allows for an assessment of the mucin content of an individual's glands prior to secretory stimulation will be used. Flow rates, mucin and protein contents of saliva, as well as mucin nascent polypeptides and mucin polysome contents of glands will be obtained. Finally, the possibility that mucin oligosaccharides undergo a processing step at secretion will be investigated. A protocol will be used that provides for carbohydrate analyses of the mucin from the same individual before and after secretion.

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Research Project (R01)
Project #
5R01DE006892-03
Application #
3220378
Study Section
Oral Biology and Medicine Study Section (OBM)
Project Start
1984-03-01
Project End
1987-02-28
Budget Start
1986-03-01
Budget End
1987-02-28
Support Year
3
Fiscal Year
1986
Total Cost
Indirect Cost
Name
University of Southern California
Department
Type
Schools of Dentistry/Oral Hygn
DUNS #
041544081
City
Los Angeles
State
CA
Country
United States
Zip Code
90033
Baughan, L W; Robertello, F J; Sarrett, D C et al. (2000) Salivary mucin as related to oral Streptococcus mutans in elderly people. Oral Microbiol Immunol 15:4-Oct
Liu, P; Denny, P A; Denny, P (2000) The effect of ageing on parenchymal cell populations in adult female mouse submandibular gland. Arch Oral Biol 45:585-92
Denny, P C; Liu, P; Denny, P A (1999) Evidence of a phenotypically determined ductal cell lineage in mouse salivary glands. Anat Rec 256:84-90
Nowroozi, N; Denny, P A; Denny, P C et al. (1998) Two gene products for beta-galactosidase are differentially expressed in the mouse salivary glands. J Craniofac Genet Dev Biol 18:51-7
Denny, P C; Ball, W D; Redman, R S (1997) Salivary glands: a paradigm for diversity of gland development. Crit Rev Oral Biol Med 8:51-75
Denny, P C; Mirels, L; Denny, P A (1996) Mouse submandibular gland salivary apomucin contains repeated N-glycosylation sites. Glycobiology 6:43-50
Denny, P C; Denny, P A; Hong-Le, N H (1995) Characterization of asparagine-linked oligosaccharides on a mouse submandibular mucin. Glycobiology 5:589-97
Bekhor, I; Wen, Y; Shi, S et al. (1994) cDNA cloning, sequencing and in situ localization of a transcript specific to both sublingual demilune cells and parotid intercalated duct cells in mouse salivary glands. Arch Oral Biol 39:1011-22
Navazesh, M; Mulligan, R A; Kipnis, V et al. (1992) Comparison of whole saliva flow rates and mucin concentrations in healthy Caucasian young and aged adults. J Dent Res 71:1275-8
Denny, P A; Hong, S H; Klauser, D K et al. (1992) Increased mucin levels in submandibular saliva from mice following repeated isoproterenol treatment. Arch Oral Biol 37:73-5

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