The long term objective of this proposal is to detect and microbiologically characterize the initial periodontal lesion. Current information on the rate of disease activity in such subjects is minimal. This investigation compares the clinical and microbiological characteristics of the initial lesion of adult periodontitis, with gingivitis and health. Clinical, microbial and immunologic features will be sought that discriminate between progressive attachment loss, gingivitis, and health.
The first aim will determine clinical features of the initial periodontal lesion in adults. Healthy and gingivitis subjects will be monitored longitudinally using electronic temperature and attachment level probes seeking sites showing significant losses in periodontal attachment level (disease activity). The gingivitis population will be enriched for active sites by screening for subjects with elevated subgingival temperatures. Comparison of clinical characteristics of sites and subjects with active lesions, with gingivitis sites from gingivitis subjects, and healthy sites from healthy subjects, should indicate which features are related to disease activity, and whether any clinical features including increased temperature are indicators for elevated risk for disease activity. To determine the etiology of the initial periodontal lesion, the predominant cultivable microbiota will be determined from disease active sites, and compared with that of gingivitis sites in gingivitis subjects, and with healthy sites in healthy subjects. Comparison of the microbiota of active lesions with gingivitis and healthy sites should indicate which species are related to progressive disease, and whether any microorganisms are useful indicators of the risk for conversion of health or gingivitis to active disease. Serum antibodies will be sought to dominant subgingival species to immunologically characterize the initial periodontal lesion. Ultimately, this information will contribute to early identification of subjects at elevated risk for onset of periodontitis, and improve our understanding of the initial stage of periodontal disease.
|Stashenko, Philip; Van Dyke, Thomas; Tully, Patrice et al. (2011) Inflammation and genetic risk indicators for early periodontitis in adults. J Periodontol 82:588-96|
|Tanner, Anne C R; Kent Jr, Ralph; Kanasi, Eleni et al. (2007) Clinical characteristics and microbiota of progressing slight chronic periodontitis in adults. J Clin Periodontol 34:917-30|
|Tanner, A C R; Paster, B J; Lu, S C et al. (2006) Subgingival and tongue microbiota during early periodontitis. J Dent Res 85:318-23|
|Tanner, Anne C R; Kent Jr, Ralph; Van Dyke, Thomas et al. (2005) Clinical and other risk indicators for early periodontitis in adults. J Periodontol 76:573-81|
|Downes, Julia; Sutcliffe, Iain; Tanner, Anne C R et al. (2005) Prevotella marshii sp. nov. and Prevotella baroniae sp. nov., isolated from the human oral cavity. Int J Syst Evol Microbiol 55:1551-5|
|Hughes, C V; Malki, G; Loo, C Y et al. (2003) Cloning and expression of alpha-D-glucosidase and N-acetyl-beta-glucosaminidase from the periodontal pathogen, Tannerella forsythensis (Bacteroides forsythus). Oral Microbiol Immunol 18:309-12|
|Paster, Bruce J; Russell, Meaghan K; Alpagot, Tamer et al. (2002) Bacterial diversity in necrotizing ulcerative periodontitis in HIV-positive subjects. Ann Periodontol 7:8-16|
|Yang, E Y; Tanner, A C R; Milgrom, P et al. (2002) Periodontal pathogen detection in gingiva/tooth and tongue flora samples from 18- to 48-month-old children and periodontal status of their mothers. Oral Microbiol Immunol 17:55-9|
|Tanner, A C R; Milgrom, P M; Kent Jr, R et al. (2002) The microbiota of young children from tooth and tongue samples. J Dent Res 81:53-7|
|Tanner, A C R; Milgrom, P M; Kent Jr, R et al. (2002) Similarity of the oral microbiota of pre-school children with that of their caregivers in a population-based study. Oral Microbiol Immunol 17:379-87|
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