Abstract

Saliva is normally inhibitory for HIV replication. One of the factors which may be involved in this is the primary defense factor in the oral cavity, namely secretory IgA. IgA is transported into secretions by secretory component (SC) synthesized by epithelial cells in the salivary glands. The expression of SC is regulated by cytokines. Since HIV infection has a profound effect on the immune system, the properties of salivary IgA antibodies of HIV infected patients will be examined including their specificity structure and function. Secondly, investigations will be initiated on the alterations of salivary cytokines or on cytokine expression by cells of the oral cavity which may influence oral manifestation of HIV infection. Third, the potential for intracellular formation of IgA anti-HIV/HIV complexes will be examined, which could lead to virus neutralization within epithelial cells in the oral cavity.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Research Project (R01)
Project #
5R01DE012146-05
Application #
6176005
Study Section
Special Emphasis Panel (ZDE1-YS (31))
Program Officer
Mangan, Dennis F
Project Start
1996-09-30
Project End
2001-07-31
Budget Start
2000-08-01
Budget End
2001-07-31
Support Year
5
Fiscal Year
2000
Total Cost
$312,116
Indirect Cost

  Institution

Name
University of Alabama Birmingham
Department
Microbiology/Immun/Virology
Type
Schools of Medicine
DUNS #
063690705
City
Birmingham
State
AL
Country
United States
Zip Code
35294

  Publications

Wu, Xueling; Hall, Stacy; Jackson, Susan (2003) Tropism-restricted neutralization by secretory IgA from parotid saliva of HIV type 1-infected individuals. AIDS Res Hum Retroviruses 19:275-81
Moore, Jennifer S; Rahemtulla, Firoz; Kent, Leigh W et al. (2003) Oral epithelial cells are susceptible to cell-free and cell-associated HIV-1 infection in vitro. Virology 313:343-53
Wu, Xueling; Jackson, Susan (2002) A longitudinal study of plasma and salivary antibodies in HIV-1 infection. Viral Immunol 15:325-35
Moore, Jennifer S; Hall, Stacy D; Jackson, Susan (2002) Cell-associated HIV-1 infection of salivary gland epithelial cell lines. Virology 297:89-97
Wu, Xueling; Jackson, Susan (2002) Plasma and salivary IgA subclasses and IgM in HIV-1-infected individuals. J Clin Immunol 22:106-15
Cummins Jr, J E; Bunn, W J; Hall, S D et al. (2001) In vitro exposure to highly cytopathic HIV-1 X4 strains increases expression of mucosa-associated integrins on CD4(+) T cells. Virology 280:262-72
Black, K P; Merrill, K W; Jackson, S et al. (2000) Cytokine profiles in parotid saliva from HIV-1-infected individuals: changes associated with opportunistic infections in the oral cavity. Oral Microbiol Immunol 15:74-81
Han, Y; Ventura, C L; Black, K P et al. (2000) Productive human immunodeficiency virus-1 infection of epithelial cell lines of salivary gland origin. Oral Microbiol Immunol 15:82-8
Jackson, S; Prince, S; Kulhavy, R et al. (2000) False positivity of enzyme-linked immunosorbent assay for measurement of secretory IgA antibodies directed at HIV type 1 antigens. AIDS Res Hum Retroviruses 16:595-602
Wu, X; Jackson, S (2000) Plasma and salivary IgG subclasses in HIV type 1 infection: evidence of both transudation and local synthesis of IgG in parotid saliva. AIDS Res Hum Retroviruses 16:1423-31