The overall goal of this proposal is to investigate the neural mechanisms underlying oral irritation produced by common irritant chemicals in foods, beverages and other consumer products. A multidisciplinary experimental approach will address the hypothesis that neurons in the trigeminal pain pathway in a rodent model signal oral irritation in a manner that correlates with human perception.
Specific aims are as follows.
Specific Aim 1. To investigate the distinct patterns of sensation elicited by different irritant chemicals in human subjects. Repetitive or continual intraoral application of certain irritants (capsaicin, concentrated NaC1) elicits irritation that progressively increases in intensity (sensitization), while irritation elicited by other irritants (nicotine menthol) declines (desensitization). We will study if additional irritant chemicals (acids, mustard oil, CO2) can be categorized as sensitizing or desensitizing. We will also investigate interactions between irritants (e.g., cross-desensitization), and if humans can qualitatively discriminate between irritants independent of taste or smell cues.
Specific Aim 2. To investigate neural mechanisms of oral irritation, by recording responses of single nociceptive neurons in subnucleus caudalis (Vc) to lingual application of irritant chemicals in rats. We will determine if the irritant-evoked Vc neuronal firing pattern correlates with the pattern of human sensory ratings. Capsaicin and other sensitizing agents are predicted to elicit a progressive increase in firing, while a progressive decline in firing is predicted for nicotine and other desensitizing agents. Additional goals are to test if """"""""chemo- selective"""""""" Vc neurons exist, and if interneurons and ascending projection neurons in Vc have similar or different properties.
Specific Aim 3. To investigate transduction mechanisms, by determining if chemically-evoked irritant sensation, or activation of Vc neurons, is reduced by specific antagonists. We will focus on neuronal nicotinic receptors, amiloride-sensitive ion channels, vanilloid (VR-1) receptors, and the Na-/H- membrane exchange pump. Vc activity will be measured by single-unit recording and by c-fog immunohistochemistry.
Specific Aim 4. To develop a rodent model of oral irritation, using a paired preference paradigm to measure consumption of water vs. an aqueous solution of capsaicin. We hypothesize that detectable concentrations of capsaicin will be rejected. This model will be used to investigate the role of the neuropeptide, substance P, in oral irritation.
Specific Aim 5. To test the hypothesis that intraoral irritants reduce human judgments of the intensity of taste qualities using a sensitive 2-alternative forced-choice paradigm. In a corresponding animal study, we will determine if responses of neurons in the n. tractus solitarius (NTS), a taste relay, are reduced by intraoral capsaicin.

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Research Project (R01)
Project #
3R01DE013685-03S1
Application #
6834507
Study Section
Integrative, Functional and Cognitive Neuroscience 8 (IFCN)
Program Officer
Kusiak, John W
Project Start
2001-08-15
Project End
2005-07-31
Budget Start
2003-08-01
Budget End
2004-07-31
Support Year
3
Fiscal Year
2004
Total Cost
$14,850
Indirect Cost
Name
University of California Davis
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
047120084
City
Davis
State
CA
Country
United States
Zip Code
95618
Akiyama, Tasuku; Ivanov, Margaret; Nagamine, Masaki et al. (2016) Involvement of TRPV4 in Serotonin-Evoked Scratching. J Invest Dermatol 136:154-160
Akiyama, Tasuku; Lerner, Ethan A; Carstens, E (2015) Protease-activated receptors and itch. Handb Exp Pharmacol 226:219-35
Klein, A H; Trannyguen, Minh; Joe, Christopher L et al. (2015) Thermosensitive transient receptor potential (TRP) channel agonists and their role in mechanical, thermal and nociceptive sensations as assessed using animal models. Chemosens Percept 8:96-108
Akiyama, Tasuku; Nguyen, Tony; Curtis, Eric et al. (2015) A central role for spinal dorsal horn neurons that express neurokinin-1 receptors in chronic itch. Pain 156:1240-6
Akiyama, T; Nagamine, M; Davoodi, A et al. (2015) Intradermal endothelin-1 excites bombesin-responsive superficial dorsal horn neurons in the mouse. J Neurophysiol 114:2528-34
Akiyama, Tasuku; Tominaga, Mitsutoshi; Takamori, Kenji et al. (2014) Roles of glutamate, substance P, and gastrin-releasing peptide as spinal neurotransmitters of histaminergic and nonhistaminergic itch. Pain 155:80-92
Klein, A H; Joe, C L; Davoodi, A et al. (2014) Eugenol and carvacrol excite first- and second-order trigeminal neurons and enhance their heat-evoked responses. Neuroscience 271:45-55
Boucher, Yves; Simons, Christopher T; Carstens, Mirela Iodi et al. (2014) Effects of gustatory nerve transection and/or ovariectomy on oral capsaicin avoidance in rats. Pain 155:814-20
Klein, Amanda H; Carstens, Mirela Iodi; Carstens, Earl (2013) Eugenol and carvacrol induce temporally desensitizing patterns of oral irritation and enhance innocuous warmth and noxious heat sensation on the tongue. Pain 154:2078-87
Akiyama, Tasuku; Tominaga, Mitsutoshi; Davoodi, Auva et al. (2013) Roles for substance P and gastrin-releasing peptide as neurotransmitters released by primary afferent pruriceptors. J Neurophysiol 109:742-8

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