This proposal continues our efforts to elucidate the genetic regulatory network underlying craniofacial development. The craniofacial skeleton consists of viscerocranium and neurocranium, which is subdivided into the calvarium/skull vault and chondrocranium/skull base. During development of the calvarium, cranial sutures serve as growth centers for skeletogenesis. Defects in suture morphogenesis resulting in premature closure are causally linked to congenital craniofacial deformities in humans. Although human genetic analyses have identified genes involved in pathogenesis of these diseases, little is known about the regulation of suture closure which is essential for development of a healthy skull. In the previously proposed investigation, we have linked the canonical Wnt pathway to calvarial development by showing that Axin2-deficient mice exhibit suture defects resembling craniosynostosis in humans. Axin2/?-catenin signaling regulates the expansion of suture stem cells and their subsequent developmental processes. Knockout of Axin2 also results in induction of the synostosis-related genes, including those encoding members of the FGF receptor family. Mutations in these genes have been linked to synostosis-related syndromes in humans and mice. We have further shown that the balance of Wnt and FGF is essential for determining the lineage commitment of suture stem cells during calvarial development. The results identify endochondral ossification caused by switching the stem cell fate as a mechanism of suture closure during development and implicate this process in craniosynostosis. Our findings have led us to propose a model in which the interplay of Wnt, FGF and BMP signaling is essential for orchestrating the calvarial morphogenetic regulatory network. In this proposal, we will continue to elucidate the mechanism underlying calvarial morphogenesis coordinately mediated by these pathways in health and disease.
Three specific aims are designed to: 1) define the role of BMP signaling as a key determinant in development of suture mesenchyme;2) elucidate the mechanism underlying the crosstalk of BMP and FGF signaling in calvarial morphogenesis;3) determine the role of Gpr177 in Wnt-mediated development of craniofacial skeleton.

Public Health Relevance

This proposal investigates the basic genetic elements controlling the formation of a healthy skull during craniofacial skeletogenesis. Using genetically modified mouse strains, we elucidate the mechanism underlying the interplay of three evolutionary conserved signal transduction pathways in normal development and pathogenesis of the skull vault. The results obtained in our study are highly relevant to the health of human development in craniofacial deformities, such as craniosynostosis and cleiocranial dysplasia, and has potentials to gain insights into therapeutic strategies for human diseases.

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Research Project (R01)
Project #
5R01DE015654-08
Application #
8667323
Study Section
Skeletal Biology Development and Disease Study Section (SBDD)
Program Officer
Scholnick, Steven
Project Start
2006-07-01
Project End
2017-05-31
Budget Start
2014-06-01
Budget End
2015-05-31
Support Year
8
Fiscal Year
2014
Total Cost
Indirect Cost
Name
University of Rochester
Department
Genetics
Type
School of Medicine & Dentistry
DUNS #
City
Rochester
State
NY
Country
United States
Zip Code
14627
Maruyama, Takamitsu; Jeong, Jaeim; Sheu, Tzong-Jen et al. (2016) Stem cells of the suture mesenchyme in craniofacial bone development, repair and regeneration. Nat Commun 7:10526
Maruyama, Eri O; Lin, Heng; Chiu, Shang-Yi et al. (2016) Extraembryonic but not embryonic SUMO-specific protease 2 is required for heart development. Sci Rep 6:20999
Zhu, Xiao-Jing; Liu, Yudong; Yuan, Xueyan et al. (2016) Ectodermal Wnt controls nasal pit morphogenesis through modulation of the BMP/FGF/JNK signaling axis. Dev Dyn 245:414-26
Das, Soumyashree; Yu, Shiyan; Sakamori, Ryotaro et al. (2015) Rab8a vesicles regulate Wnt ligand delivery and Paneth cell maturation at the intestinal stem cell niche. Development 142:2147-62
Zhu, Xiao-Jing; Liu, YuDong; Dai, Zhong-Min et al. (2014) BMP-FGF signaling axis mediates Wnt-induced epidermal stratification in developing mammalian skin. PLoS Genet 10:e1004687
Li, T-F; Yukata, K; Yin, G et al. (2014) BMP-2 induces ATF4 phosphorylation in chondrocytes through a COX-2/PGE2 dependent signaling pathway. Osteoarthritis Cartilage 22:481-9
McGee-Lawrence, Meghan E; Carpio, Lomeli R; Bradley, Elizabeth W et al. (2014) Runx2 is required for early stages of endochondral bone formation but delays final stages of bone repair in Axin2-deficient mice. Bone 66:277-86
Sakamori, Ryotaro; Yu, Shiyan; Zhang, Xiao et al. (2014) CDC42 inhibition suppresses progression of incipient intestinal tumors. Cancer Res 74:5480-92
McGee-Lawrence, Meghan E; Li, Xiaodong; Bledsoe, Krista L et al. (2013) Runx2 protein represses Axin2 expression in osteoblasts and is required for craniosynostosis in Axin2-deficient mice. J Biol Chem 288:5291-302
Zhu, XiaoJing; Zhao, Pan; Liu, YuDong et al. (2013) Intra-epithelial requirement of canonical Wnt signaling for tooth morphogenesis. J Biol Chem 288:12080-9

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