Human postnatal dental stem cells such as dental pulp stem cells (DPSCs) and stem cells from root apical papilla (SCAPs) are unique precursor populations isolated from dental tissues based on the primary characteristics of bone marrow mesenchymal stem cells (MSCs). Like bone marrow MSCs, dental stem cells are self-renewing, multipotent, and clonogenic. They can be induced to differentiate into odontoblast- or osteoblast-like cells and form mineralized nodules in vitro. When implanted into immunodeficient mice, dental stem cells can form dentin- or cementum-like mineralized tissues or related craniofacial structures. Hence dental stem cells may present promising prospects for tooth regeneration and tissue repair. However, currently, the molecular regulation of their differentiation is poorly understood. Histone demethylases are newly-identified enzymes for removing histone methyl markers associated with gene activation or silencing. While they have been implicated in developmental processes and human diseases, it is largely unknown whether and how histone demethylases play a critical role in regulating dental stem cell differentiation. By studying oculofacialcardiodental syndrome (OFCD), a rare human genetic disorder characterized by teeth with extremely long roots (radiculomegaly), we unexpectedly discovered that the transcription co-repressor BCOR (Bcl-6 co-repressor) epigenetically regulates dental stem cell function and differentiation via histone demethylases. In this competing renewal, we hypothesize that histone epigenetic modification plays an important role in the regulation of dental stem cell function and differentiation. To test our hypothesis, we propose three specific aims.
In Aim 1, we will explore whether and how BCOR epigenetically represses dental stem cell differentiation by histone modification.
In Aim 2, we will determine whether and how BCOR mutation epigenetically de-represses gene transcription and thereby promotes dental stem cell differentiation. These two aims will augment our current work and further define how BCOR mutation promotes dental stem cell differentiation in a pathological condition.
In Aim 3, we will explore whether and how a newly identified histone demethylase JMJD3 (JmjC domain-containing 3) promotes gene expression and controls dental stem cell differentiation in healthy conditions. By studying both normal and abnormal dental stem cells, our results may provide new insights into the molecular biology of human dental stem cells. Moreover, as demethylases, being enzymes, can be readily targeted by small molecule inhibitors, our work may help to develop novel strategies for promoting dental and craniofacial tissue regeneration and repair.

Public Health Relevance

Human postnatal dental stem cells are unique precursor populations which are isolated tooth. These cells are capable of differentiating into dentin/bone-like forming cells. When implanted into immunodeficient mice, dental stem cells can form dentin- or cement-like mineralized tissues. Although dental stem cells may present promising application prospects in tooth regeneration and repair, currently, molecular regulation of their fate is poorly understood. Histone demethylases are newly-identified enzymes that remove histone methyl marks associated with gene activation or repression. While they have been implicated in developmental processes and human diseases, it is largely unknown whether and how histone demethylases play a critical role in dental stem cell function. In this application, we propose to examine how chromatin modification by histone demethylases regulates gene expression and dental stem cell differentiation using molecular and genetic approaches. Our results may provide new insights into the molecular biology of human dental stem cells. Moreover, because demethylases, being enzymes, can be readily targeted by small molecule inhibitors, our work may help to develop novel strategies for improving dental and craniofacial tissue regeneration and repair.

Agency
National Institute of Health (NIH)
Type
Research Project (R01)
Project #
5R01DE016513-11
Application #
8640912
Study Section
Special Emphasis Panel (ZRG1)
Program Officer
Lumelsky, Nadya L
Project Start
Project End
Budget Start
Budget End
Support Year
11
Fiscal Year
2014
Total Cost
Indirect Cost
Name
University of California Los Angeles
Department
Dentistry
Type
Schools of Dentistry/Oral Hygn
DUNS #
City
Los Angeles
State
CA
Country
United States
Zip Code
90095
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