The overall objective of this proposed research is to develop polarization sensitive optical coherence tomography (PS-OCT) as a non-invasive optical device for the accurate and early clinical assessment of tooth demineralization and remineralization. The central hypothesis underlying this proposal is that the depth-resolved changes in the magnitude of light scattering of dental hard tissues at 1310-nm, that occur upon demineralization and remineralization, can be imaged and quantified in vivo using PS-OCT and that those changes can be correlated with the structural changes and relative mineral loss measured using polarized-light microscopy and microradiography. The overall objectives of this proposal will be achieved through the following specific aims: (1) To test the hypothesis that the lesion volume and reflectivity from that volume can be automatically assessed in simulated in vitro caries models and natural lesions on extracted teeth using a high speed PS-OCT scanning system;(2) To test the hypothesis that PS-OCT can be used to longitudinally monitor demineralization and the inhibition of demineralization by fluoride in an extended clinical study;(3) To test the hypothesis that PS-OCT can be used to detect changes in the structure and severity of existing lesions after exposure to a remineralization agent in vivo. These studies will further validate PS-OCT as a clinical tool for the accurate and early clinical assessment of tooth demineralization and remineralization. This is expected to lead to the clinical implementation of PS-OCT for the assessment and monitoring of the activity and severity of early carious lesions without the use of ionizing radiation, leading to an increased efficiency of caries clinical trials and a marked reduction in their scope and cost.

Public Health Relevance

The overall objective of this proposed research is to develop polarization sensitive optical coherence tomography (PS-OCT) as a non-invasive optical device for the accurate and early clinical assessment of tooth demineralization and remineralization. This is expected to lead to the clinical implementation of PS-OCT for the assessment and monitoring of the activity and severity of early carious lesions without the use of ionizing radiation, leading to an increased efficiency of caries clinical trials and a marked reduction in their scope and cost.

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Research Project (R01)
Project #
5R01DE017869-08
Application #
8678710
Study Section
Special Emphasis Panel (ZRG1)
Program Officer
Drummond, James
Project Start
2006-08-15
Project End
2015-07-31
Budget Start
2014-08-01
Budget End
2015-07-31
Support Year
8
Fiscal Year
2014
Total Cost
Indirect Cost
Name
University of California San Francisco
Department
Dentistry
Type
Schools of Dentistry/Oral Hygn
DUNS #
City
San Francisco
State
CA
Country
United States
Zip Code
94143
Tom, Henry; Chan, Kenneth H; Darling, Cynthia L et al. (2016) Near-IR image-guided laser ablation of demineralization on tooth occlusal surfaces. Lasers Surg Med 48:52-61
Kim, Jin Wan; Chan, Kenneth H; Fried, Daniel (2016) Evaluation of enamel surface modification using PS-OCT after laser treatment to increase resistance to demineralization. Proc SPIE Int Soc Opt Eng 9692:
Lee, Robert C; Staninec, Michal; Le, Oanh et al. (2016) Infrared Methods for Assessment of the Activity of Natural Enamel Caries Lesions. IEEE J Sel Top Quantum Electron 22:
Lee, Robert C; Darling, Cynthia L; Fried, Daniel (2016) Assessment of remineralized dentin lesions with thermal and near-infrared reflectance imaging. Proc SPIE Int Soc Opt Eng 9692:
Kang, Hobin; Darling, Cynthia L; Fried, Daniel (2016) Use of an optical clearing agent to enhance the visibility of subsurface structures and lesions from tooth occlusal surfaces. J Biomed Opt 21:081206
Simon, Jacob C; A Lucas, Seth; Lee, Robert C et al. (2016) Near-infrared imaging of secondary caries lesions around composite restorations at wavelengths from 1300-1700-nm. Dent Mater 32:587-95
Tam, Wilson; Lee, Robert C; Lin, Brent et al. (2016) Assessment of simulated lesions on primary teeth with near-infrared imaging. Proc SPIE Int Soc Opt Eng 9692:
Lee, Robert C; Darling, Cynthia L; Staninec, Michal et al. (2016) Activity assessment of root caries lesions with thermal and near-IR imaging methods. J Biophotonics :
Kang, Hobin; Darling, Cynthia L; Fried, Daniel (2016) Enhancement of OCT images with vinyl polysiloxane (VPS). Proc SPIE Int Soc Opt Eng 9692:
Kang, Hobin; Darling, Cynthia L; Tom, Henry et al. (2015) Enhanced detection of dentinal lesions in OCT images using the RKT transformation. Proc SPIE Int Soc Opt Eng 9306:

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