Streptococcus sanguinis is a common pathogen in endocarditis and a pioneer colonizer of human teeth. In heart tissues, S. sanguinis can cause infective endocarditis, but in the mouth, S. sanguinis is considered a benign or even a beneficial bacterium because the presence of S. sanguinis delays caries caused by S. mutans. A putative global regulator, Mgs, was identified in the recently completed S. sanguinis genome. Genomic analysis has revealed that the Mgs coding gene is a horizontally transferred gene. Real-time quantitative PCR experiments suggest that Mgs regulates several putative virulence genes. The hypothesis is that Mgs is a transcription regulator involved in virulence gene expression in S. sanguinis. The objective of this application is to understand the mechanism of Mgs-mediated gene regulation in endocarditis and its effects on S. sanguinis in the oral community. We propose 1) to identify Mgs-regulated genes;2) to identify Mgs DNA-binding sites and its binding domains;3) to identify relationships among Mgs regulated proteins in silico;and 4) to identify Mgs regulated protein expressions and functions. These analyses will improve our understanding at the molecular level of this regulator and identify new targets for development of strategies, such as chemo- or immunotherapy, against streptococcal endocarditis. PUBLIC HEALTH SIGNIFICANCE: Streptococcus sanguinis is a common pathogen in endocarditis and a pioneer colonizer of human teeth. Endocarditis is a serious, often fatal, infection of the heart. We have compared the S. sanguinis genome with other streptococcal genomes and identified a global regulator, Mgs. We propose to examine Mgs regulation, its DNA binding site, and its function in endocarditis for developing chemotherapeutic or immunotherapeutic strategies against streptococcal endocarditis.

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Research Project (R01)
Project #
5R01DE018138-04
Application #
8015200
Study Section
Special Emphasis Panel (ZRG1-MOSS-E (02))
Program Officer
Lunsford, Dwayne
Project Start
2008-03-17
Project End
2013-01-31
Budget Start
2011-02-01
Budget End
2012-01-31
Support Year
4
Fiscal Year
2011
Total Cost
$312,282
Indirect Cost
Name
Virginia Commonwealth University
Department
Dentistry
Type
Schools of Dentistry
DUNS #
105300446
City
Richmond
State
VA
Country
United States
Zip Code
23298
Ge, Xiuchun; Shi, Xiaoli; Shi, Limei et al. (2016) Involvement of NADH Oxidase in Biofilm Formation in Streptococcus sanguinis. PLoS One 11:e0151142
Chen, Lei; Ge, Xiuchun; Xu, Ping (2015) Identifying essential Streptococcus sanguinis genes using genome-wide deletion mutation. Methods Mol Biol 1279:15-23
Stone, Victoria N; Parikh, Hardik I; El-rami, Fadi et al. (2015) Identification of Small-Molecule Inhibitors against Meso-2, 6-Diaminopimelate Dehydrogenase from Porphyromonas gingivalis. PLoS One 10:e0141126
Xu, Ping; Gunsolley, John (2014) Application of metagenomics in understanding oral health and disease. Virulence 5:424-32
Evans, Karra; Stone, Victoria; Chen, Lei et al. (2014) Systematic study of genes influencing cellular chain length in Streptococcus sanguinis. Microbiology 160:307-15
Trihn, My; Ge, Xiuchun; Dobson, Alleson et al. (2013) Two-component system response regulators involved in virulence of Streptococcus pneumoniae TIGR4 in infective endocarditis. PLoS One 8:e54320
Ge, Xiuchun; Rodriguez, Rafael; Trinh, My et al. (2013) Oral microbiome of deep and shallow dental pockets in chronic periodontitis. PLoS One 8:e65520
Ge, Xiuchun; Xu, Ping (2012) Genome-wide gene deletions in Streptococcus sanguinis by high throughput PCR. J Vis Exp :
Chen, Lei; Ge, Xiuchun; Wang, Xiaojing et al. (2012) SpxA1 involved in hydrogen peroxide production, stress tolerance and endocarditis virulence in Streptococcus sanguinis. PLoS One 7:e40034
Chen, Lei; Ge, Xiuchun; Dou, Yuetan et al. (2011) Identification of hydrogen peroxide production-related genes in Streptococcus sanguinis and their functional relationship with pyruvate oxidase. Microbiology 157:13-20

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