Because of the formation and subsequent sloughing off of microbial biofilms from the inner surfaces of plastic tubing, dental unit water is heavily contaminated with microorganisms. These microorganisms pose a potentially significant threat to both dental health care workers and dental patients, particularly those who are medically compromised or immunocompromised. The long-range goal of this project is to use N-halamine- based rechargeable antimicrobial tubing to control the formation of dental unit waterline biofilms.
The specific aims of the proposed research are to: (1) covalently bind "self-recharging" N-halamines onto plastic tubing;(2) characterize the mechanical properties, antimicrobial activity, anti-adherent function, and rechargeability of the new tubes;(3) evaluate the biofilm-controlling function and safety of the new tubes in model dental water delivery systems, and (4) provide preliminary data on the cost of the new approach. To achieve these goals, the first part of the proposed research will optimize the grafting reactions to covalently bind both amide and amine N-halamine precursors into plastic tubing currently used in outpatient dental clinic waterlines, so as to introduce self-recharging antimicrobial effects into the tubing and achieve long-lasting biofilm-control (e.g., longer than 1 month). This will be followed by a series of studies to provide detailed information on the mechanical properties, antimicrobial/anti-adherent activity, rechargeability, and effects of recharging on the mechanical properties of the new tubes. Then the new tubes will be evaluated with mixed species of water bacteria in model dental water delivery systems to determine the efficacy and safety of the new approach in controlling DUWL biofilms. Finally, the cost of the tubes will be estimated, as a first step in evaluating the cost-effectiveness of the new approach. If successful, the rechargeable antimicrobial tubing approach will provide an innovative solution to the biofilm problem in dental unit water delivery systems. Further, the antimicrobial plastic tube materials to be developed in this study may find applications in other clinically important surfaces. All these will make significant contributions to a better and safer healthcare environment.

Public Health Relevance

The rechargeable antimicrobial tubing technology will provide an innovative solution to the biofilm problem in DUWLs and will thus have a significant impact on the quality of dental care. In addition, the antimicrobial plastic tubing materials to be developed in this study may also find applications in hospital showers, respiratory care equipment, water distribution systems, and other clinically important surfaces to make significant contributions to a better and safer healthcare environment. If the proposed study indicates that the approach is safe and effective, in-use evaluation of the new tubing will be performed in further studies to improve dental water quality and benefit the dental/medical community and the general public.

Agency
National Institute of Health (NIH)
Type
Research Project (R01)
Project #
5R01DE018707-07
Application #
8685761
Study Section
Oral, Dental and Craniofacial Sciences Study Section (ODCS)
Program Officer
Drummond, James
Project Start
Project End
Budget Start
Budget End
Support Year
7
Fiscal Year
2014
Total Cost
Indirect Cost
Name
University of Massachusetts Lowell
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
City
Lowell
State
MA
Country
United States
Zip Code
01854
Porteous, Nuala; Sun, Yuyu; Dang, Shichien et al. (2013) A comparison of 2 laboratory methods to test dental unit waterline water quality. Diagn Microbiol Infect Dis 77:206-8
Sun, Xinbo; Cao, Zhengbing; Porteous, Nuala et al. (2012) An N-halamine-based rechargeable antimicrobial and biofilm controlling polyurethane. Acta Biomater 8:1498-506
Luo, Jie; Porteous, Nuala; Sun, Yuyu (2011) Rechargeable biofilm-controlling tubing materials for use in dental unit water lines. ACS Appl Mater Interfaces 3:2895-903
Sun, Xinbo; Cao, Zhengbing; Porteous, Nuala et al. (2010) Amine, Melamine, and Amide N-Halamines as Antimicrobial Additives for Polymers. Ind Eng Chem Res 49:11206-11213
Sun, Xinbo; Zhang, Lifeng; Cao, Zhengbing et al. (2010) Electrospun composite nanofiber fabrics containing uniformly dispersed antimicrobial agents as an innovative type of polymeric materials with superior antimicrobial efficacy. ACS Appl Mater Interfaces 2:952-6