Engineering vascularized bone tissues by microfabrication and scaffolding Repair of craniofacial and other large bone defects remains a significant clinical problem. Often bone grafts are required for reconstructive skeletal procedures to aid fracture healing, bone fusion, implant integration and repair of skeletal defects, but the use of allograft bone is limited by a high failure rate. Tissue engineered constructs are promising substitutes. The major challenge of bone tissue engineering is the formation of a complete vascular network capable of delivering oxygen and nutrients and removing waste products that limits the maximum effective size of tissue engineered constructs. Most tissue engineering approaches rely on the self-assembly of cells to recreate functional vascularity on biodegradable scaffolds, but it rarely occurs. We therefore propose to construct bone tissues having a vascular network by (1) microfabricating and optimizing a vascular network with biomimetic complexity;(2) optimizing a synthetic bony environment to support the function of the microfabricated vascular networks;and (3) validating enhanced functionality of the developed vascularized bone tissue constructs in vivo. The success of this project will significantly advance the paradigm of vascular networks in engineered tissues by the creation of a vascularized bone construct prototype, with a long term goal of a new treatment in large bone defect repair in humans.
Repair of craniofacial and other large bone defects remains a significant clinical challenge. This project is expected to generate a new paradigm in bone tissue engineering by enabling the formation of a complete vascular network at a large scale by microfabrication and scaffolding.
|Miri, Amir K; Nieto, Daniel; Iglesias, Luis et al. (2018) Microfluidics-Enabled Multimaterial Maskless Stereolithographic Bioprinting. Adv Mater 30:e1800242|
|Liu, Wanjun; Zhong, Zhe; Hu, Ning et al. (2018) Coaxial extrusion bioprinting of 3D microfibrous constructs with cell-favorable gelatin methacryloyl microenvironments. Biofabrication 10:024102|
|Seo, Jungmok; Shin, Jung-Youn; Leijten, Jeroen et al. (2018) High-throughput approaches for screening and analysis of cell behaviors. Biomaterials 153:85-101|
|Kawai, Toshiyuki; Shanjani, Yaser; Fazeli, Saba et al. (2018) Customized, degradable, functionally graded scaffold for potential treatment of early stage osteonecrosis of the femoral head. J Orthop Res 36:1002-1011|
|Ker, Dai Fei Elmer; Wang, Dan; Behn, Anthony William et al. (2018) Functionally Graded, Bone- and Tendon-Like Polyurethane for Rotator Cuff Repair. Adv Funct Mater 28:|
|Saghazadeh, Saghi; Rinoldi, Chiara; Schot, Maik et al. (2018) Drug delivery systems and materials for wound healing applications. Adv Drug Deliv Rev 127:138-166|
|Shin, Su Ryon; Migliori, Bianca; Miccoli, Beatrice et al. (2018) Electrically Driven Microengineered Bioinspired Soft Robots. Adv Mater 30:|
|Zhang, Yu Shrike; Yue, Kan; Aleman, Julio et al. (2017) 3D Bioprinting for Tissue and Organ Fabrication. Ann Biomed Eng 45:148-163|
|Leijten, Jeroen; Seo, Jungmok; Yue, Kan et al. (2017) Spatially and Temporally Controlled Hydrogels for Tissue Engineering. Mater Sci Eng R Rep 119:1-35|
|Santa Maria, Peter Luke; Gottlieb, Peter; Santa Maria, Chloe et al. (2017) Functional Outcomes of Heparin-Binding Epidermal Growth Factor-Like Growth Factor for Regeneration of Chronic Tympanic Membrane Perforations in Mice. Tissue Eng Part A 23:436-444|
Showing the most recent 10 out of 147 publications