The bioengineering aspect of this research originates from Wolff's law which explains bone adaptation due to mechanical loads at an organ-level. At a tissue- and a cell-level, local strains trigger mechanobiological events and are responsible for maintaining a uniform functional PDL-space. However, a uniform PDL-space can shift toward a nonuniform PDL-space due to aberrations in strains at the PDL-bone and PDL-cementum interfaces as a result of altered mechanobiology. We wil test cell- and tissue-level responses that could cause an increase or decrease in PDL-space and the accompanying gradients that result from a change in function. We will investigate:
Aim 1) that in situ the surfaces of bone and tooth are originally conforming and with application of mechanical load can induce localized compressive and tensile strains within the bone-tooth complex. Changes in PDL-space and resulting strains will be evaluated using a loading device coupled to a Micro X-ray computed tomography (?-XCT) followed by digital image correlation (DIC) of virtual sections taken at no load and loaded conditions;
Aim 2) that in vivo the mechanobiologically active compression and tension sites promote biochemical changes through expression of genes and matrix proteins, causing resorption and formation of bone and cementum resulting in a widened or a narrowed PDL-space. The spatiotemporal outcomes of mechanosensitive genes and protein expressions promoting mineral formation at the tension sites, resorption at the compression sites, and responsible for maintaining tissue and interface integrity will be mapped by using fluorescence based immunohistochemistry;
Aim 3) that biochemically altered regions identified in Aim 2 cause measurable changes in structure, chemical composition and mechanical properties of bone, cementum, PDL (e.g. tensile strains induce bone formation).
This aim i ncludes mapping the shifts in gradients of the PDL-bone and PDL-cementum interfaces. Spatiotemporal changes in local structure, chemical composition and mechanical properties of wet PDL-bone, PDL-cementum, cementum, bone, and PDL using state-of-the-art high resolution microscopy and spectroscopy equipment will be mapped;
Aim 4). the functionally adapted systems in situ have an altered response to mechanical loads. Load-displacement curves and correlating strains fields in adapted complexes of 2nd molar from freshly harvested experimental groups and controls (as in Aim 1) will be evaluated and compared. Studies will use in situ loading, ?-XCT, and DIC as in Aim 1.
The proposed translational research is based on our incomplete understanding of how PDL-space adapts to functional loads. Local functional adaptations can be unfavorable outcomes with an increased or a decreased range of tooth motion due to root and/or bone resorption or formation resulting in a widened (tooth loosening) or narrowed PDL-space (ankylosis). Such adapted sites could act as the local stress points inducing positive feedback when the complex is subjected to implants and/or periodontitis, normal or therapeutic loads (orthodontic braces) subsequently impairing function.
|Kurylo, Michael P; Grandfield, Kathryn; Marshall, Grayson W et al. (2016) Effect of proteoglycans at interfaces as related to location, architecture, and mechanical cues. Arch Oral Biol 63:82-92|
|Cao, Zhengguo; Liu, Rubing; Zhang, Hua et al. (2015) Osterix controls cementoblast differentiation through downregulation of Wnt-signaling via enhancing DKK1 expression. Int J Biol Sci 11:335-44|
|Zhang, Hua; Jiang, Yong; Qin, Chunlin et al. (2015) Essential role of osterix for tooth root but not crown dentin formation. J Bone Miner Res 30:742-6|
|Grandfield, Kathryn; Herber, Ralf Peter; Chen, Ling et al. (2015) Strain-guided mineralization in the bone-PDL-cementum complex of a rat periodontium. Bone Rep 3:20-31|
|Du, Jing; Lee, Ji-Hyun; Jang, Andrew T et al. (2015) Biomechanics and strain mapping in bone as related to immediately-loaded dental implants. J Biomech 48:3486-94|
|Ren, Yinshi; Han, Xianglong; Ho, Sunita P et al. (2015) Removal of SOST or blocking its product sclerostin rescues defects in the periodontitis mouse model. FASEB J 29:2702-11|
|Jang, Andrew T; Merkle, Arno P; Fahey, Kevin P et al. (2015) Multiscale biomechanical responses of adapted bone-periodontal ligament-tooth fibrous joints. Bone 81:196-207|
|Lee, J-H; Pryce, B A; Schweitzer, R et al. (2015) Differentiating zones at periodontal ligament-bone and periodontal ligament-cementum entheses. J Periodontal Res 50:870-80|
|Djomehri, Sabra I; Candell, Susan; Case, Thomas et al. (2015) Mineral density volume gradients in normal and diseased human tissues. PLoS One 10:e0121611|
|Jang, Andrew T; Lin, Jeremy D; Choi, Ryan M et al. (2014) Adaptive properties of human cementum and cementum dentin junction with age. J Mech Behav Biomed Mater 39:184-96|
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