Periodontitis is a polymicrobial infection that causes destruction of tooth-supporting structures and results in tooth loss. 41.9% of periodontitis is attributable to smoking;and with 1.2 billion adults worldwide currently smoking, this disease presents a global public health issue. Subgingival bacteria are known to play an important role in disease pathogenesis;however, the effects of smoking on this community are poorly understood. Our goal is to test the hypothesis that smoking preferentially enriches this polymicrobial ecosystem for pathogenic organisms at the expense of health-compatible species. Our strategy is to combine unique, large-scale clinical studies with robust, high-throughput molecular assays, computational phylogenetics and bioinformatics for a comprehensive exploration of the subgingival microbiome. This approach will bridge the gap between clinical outcome-based studies and in vitro investigations using bacterial cultures and artificially developed biofilms. We will initially combine a cross-sectional study design with deep sequencing of 16S rRNA genes to identify bacteria associated with smoking-related periodontitis. We will then explore the compositional responses of the subgingival biofilm to smoking cessation by combining a longitudinal clinical study with deep sequencing. We will use a targeted, quantitative molecular assay to explore the salivary microbiome for disease markers and predictors. The proposed studies will bring us closer to understanding the role of smoking in the pathogenesis of periodontitis, provide biologically-validated timelines for administering periodontal therapy to patients following smoking cessation, and develop salivary indicators or predictors of disease.

Public Health Relevance

These studies represent the first comprehensive approach to examine the association between smoking and the subgingival microbial community as well as the response of this community to smoking cessation. Identification of putative pathogens and health- associated bacteria in this high-risk population will bring us closer to understanding the role of smoking in the pathogenesis of periodontitis, provide biologically-validated timelines for administering periodontal therapy to patients following smoking cessation, and develop salivary indicators or predictors of disease.

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Research Project (R01)
Project #
1R01DE022579-01
Application #
8273549
Study Section
Special Emphasis Panel (ZRG1-MOSS-S (03))
Program Officer
Lunsford, Dwayne
Project Start
2012-04-13
Project End
2017-01-31
Budget Start
2012-04-13
Budget End
2013-01-31
Support Year
1
Fiscal Year
2012
Total Cost
$381,250
Indirect Cost
$131,250
Name
Ohio State University
Department
Dentistry
Type
Schools of Dentistry
DUNS #
832127323
City
Columbus
State
OH
Country
United States
Zip Code
43210
Mason, Matthew R; Preshaw, Philip M; Nagaraja, Haikady N et al. (2015) The subgingival microbiome of clinically healthy current and never smokers. ISME J 9:268-72
Mason, Matthew R; Nagaraja, Haikady N; Camerlengo, Terry et al. (2013) Deep sequencing identifies ethnicity-specific bacterial signatures in the oral microbiome. PLoS One 8:e77287
Dabdoub, S M; Tsigarida, A A; Kumar, P S (2013) Patient-specific analysis of periodontal and peri-implant microbiomes. J Dent Res 92:168S-75S