There is an unmet need for a quick, reliable method to assess pulpal blood flow. Our long-term goal is to develop a quick, inexpensive, reliable method to enable dentists and endodontists to (i) assess pulpal vitality, (ii) make an informed decision on the appropriate treatment plan, and (iii) monitor treatment response for each individual tooth. Having determined the feasibility of dental Laser Speckle Imaging (dLSI)-based pulpal diagnosis in our preliminary studies, the overall objective of this application is to quantiy the performance of the dLSI method developed jointly by the PIs to assess pulpal blood flow and viability in a quick repeatable fashion. Our central hypothesis is that dLSI measurement of pulpal blood flow results in a positive and negative predictive value of pulpal vitality that excees the values found with either an Electrical Pulp Tester (EPT) or thermal tests. The rationale that underlies the proposed research is that we expect to demonstrate that dLSI is a reliable in-vivo method to assess pulpal vitality. Guided by strong preliminary data, we will test the hypothesis by pursuing the following two Specific Aims: 1) Develop second-generation, clinic-friendly dLSI instrument, and 2) Determine the positive and negative predictive values associated with in-vivo dLSI, EPT, and thermal tests, to assess pulpal vitality. Under the first aim, we will couple computational modeling with our extensive previous experience with clinical LSI instrument design, to develop a second- generation dLSI instrument that is designed for use in the oral cavity. Under the second aim, we will deploy this dLSI instrument to a nearby endodontic clinic to perform a clinical study and assess the capabilities of dLSI for pulpal vitality testing. The approach is innovative, in our opinion, because we propose development of an imaging approach and technology that is substantially different from that used in the oral cavity by past investigators. The proposed research is significant, because it is a first step towards development of a simple, low-cost, quick method that enables dentists and endodontists finally to assess accurately pulpal health status.

Public Health Relevance

The predominant cause of dental pain is pulpal inflammation and necrosis. Clinicians lack an inexpensive, reliable in-vivo tool for testing pulpal vitality and treatment response. The proposed research supports the NIDCR mission by providing clinicians with improved tools to detect and assess pulpal vitality for better endodontic management.

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Research Project (R01)
Project #
1R01DE022831-01A1
Application #
8506298
Study Section
Oral, Dental and Craniofacial Sciences Study Section (ODCS)
Program Officer
Atkinson, Jane C
Project Start
2013-03-07
Project End
2016-02-29
Budget Start
2013-03-07
Budget End
2014-02-28
Support Year
1
Fiscal Year
2013
Total Cost
$337,087
Indirect Cost
$112,087
Name
University of California Irvine
Department
None
Type
Organized Research Units
DUNS #
046705849
City
Irvine
State
CA
Country
United States
Zip Code
92697
Regan, Caitlin; Ramirez-San-Juan, Julio C; Choi, Bernard (2014) Photothermal laser speckle imaging. Opt Lett 39:5006-9
Ramirez-San-Juan, J C; Ramos-Garcia, R; Martinez-Niconoff, G et al. (2014) Simple correction factor for laser speckle imaging of flow dynamics. Opt Lett 39:678-81
Ramirez-San-Juan, Julio C; Regan, Caitlin; Coyotl-Ocelotl, Beatriz et al. (2014) Spatial versus temporal laser speckle contrast analyses in the presence of static optical scatterers. J Biomed Opt 19:106009