Abstract

The objective of our research is to determine the role of neuropeptides in the regulation of neuroendocrine secretion and smooth muscle activity in the gut. Initial work on a prototypical neuropeptide, VIP, will now be extended to the major neuropeptides of the myenteric and submucosal plexuses: somatostatin, CCK-OP, methionine- and leucine-enkephalin, substance P and bombesin. Smooth muscle cells will be isolated from the longitudinal and circular smooth muscle of the intestine of the guinea pig and examined for the presence of specific receptors to neuropeptides. Receptors will be characterized in terms of mechnical activity, messenger systems (Ca++ fluxes and cAMP accumulation) and binding sites (radioiodinated ligands). The release of neuropeptides will be examined in muscle strips, vascularly perfused intestine and in isolated segments of fresh and cultured neural plexus. The regulation of an endocrine peptide (gastrin) by a paracrine peptide (somatostatin) and by cholinergic and peptidergic intramural neurons will be investigated in the vascularly perfused stomach of the rat.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK015564-16
Application #
3225415
Study Section
(SSS)
Project Start
1979-04-01
Project End
1988-03-31
Budget Start
1987-04-01
Budget End
1988-03-31
Support Year
16
Fiscal Year
1987
Total Cost
Indirect Cost

  Institution

Name
Virginia Commonwealth University
Department
Type
Schools of Medicine
DUNS #
City
Richmond
State
VA
Country
United States
Zip Code
23298

  Publications

Mahavadi, Sunila; Grider, John R; Murthy, Karnam S (2018) Muscarinic m2 receptor-mediated actin polymerization via PI3 kinase ? and integrin-linked kinase in gastric smooth muscle. Neurogastroenterol Motil :e13495
Mahavadi, Sunila; Nalli, Ancy D; Wang, Hongxia et al. (2018) Regulation of gastric smooth muscle contraction via Ca2+-dependent and Ca2+-independent actin polymerization. PLoS One 13:e0209359
May, Alexander T; Crowe, Molly S; Blakeney, Bryan A et al. (2018) Identification of expression and function of the glucagon-like peptide-1 receptor in colonic smooth muscle. Peptides 112:48-55
Blakeney, Bryan A; Crowe, Molly S; Mahavadi, Sunila et al. (2018) Branched Short-Chain Fatty Acid Isovaleric Acid Causes Colonic Smooth Muscle Relaxation via cAMP/PKA Pathway. Dig Dis Sci :
Parikh, Jay; Zemljic-Harpf, Alice; Fu, Johnny et al. (2017) Altered Penile Caveolin Expression in Diabetes: Potential Role in Erectile Dysfunction. J Sex Med 14:1177-1186
Zhang, Yonggang; Li, Fang; Xiao, Xiao et al. (2017) Regulator of G protein signaling 4 is a novel target of GATA-6 transcription factor. Biochem Biophys Res Commun 483:923-929
Zhang, Yonggang; Li, Fang; Wang, Hong et al. (2016) Immune/Inflammatory Response and Hypocontractility of Rabbit Colonic Smooth Muscle After TNBS-Induced Colitis. Dig Dis Sci 61:1925-40
Liu, Miao; Shen, Shanwei; Kendig, Derek M et al. (2015) Inhibition of NMDAR reduces bladder hypertrophy and improves bladder function in cyclophosphamide induced cystitis. J Urol 193:1676-83
Rajagopal, Senthilkumar; Nalli, Ancy D; Kumar, Divya P et al. (2015) Cytokine-induced S-nitrosylation of soluble guanylyl cyclase and expression of phosphodiesterase 1A contribute to dysfunction of longitudinal smooth muscle relaxation. J Pharmacol Exp Ther 352:509-18
Mahavadi, Sunila; Nalli, Ancy D; Kumar, Divya P et al. (2014) Cytokine-induced iNOS and ERK1/2 inhibit adenylyl cyclase type 5/6 activity and stimulate phosphodiesterase 4D5 activity in intestinal longitudinal smooth muscle. Am J Physiol Cell Physiol 307:C402-11

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