DNA probes for individual gene products of both the endocrine pancreas (insulin and potentially glucagon) and the exocrine pancreas (several digestive enzymes including amylase) have been prepared by reverse transcription of mRNA and subsequent cloning in E. coli X1776. We propose to utilize the cloned structural gene sequences to probe the organization and expression of the endocrine and exocrine specific genes. The cloned sequences will be used to isolate (by cloning in E. coli X1776) insulin and amylase gene sequences from rat nuclear DNA. The isolaed genes will permit the study of the sequence organization of the structural genes and their contiguous DNA regions. The cloned structural genes will also be used to study changes in their expression during embryonic development as well as alteration in expression accompanying pathogenic states.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK021344-21
Application #
6164509
Study Section
Endocrinology Study Section (END)
Program Officer
Sato, Sheryl M
Project Start
1980-12-01
Project End
2002-02-28
Budget Start
2000-03-01
Budget End
2001-02-28
Support Year
21
Fiscal Year
2000
Total Cost
$235,065
Indirect Cost
Name
University of California San Francisco
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
073133571
City
San Francisco
State
CA
Country
United States
Zip Code
94143
Krentz, Nicole A J; van Hoof, Dennis; Li, Zhongmei et al. (2017) Phosphorylation of NEUROG3 Links Endocrine Differentiation to the Cell Cycle in Pancreatic Progenitors. Dev Cell 41:129-142.e6
Pappalardo, Zachary; Gambhir Chopra, Deeksha; Hennings, Thomas G et al. (2017) A Whole-Genome RNA Interference Screen Reveals a Role for Spry2 in Insulin Transcription and the Unfolded Protein Response. Diabetes 66:1703-1712
Berger, Miles; Scheel, David W; Macias, Hector et al. (2015) G?i/o-coupled receptor signaling restricts pancreatic ?-cell expansion. Proc Natl Acad Sci U S A 112:2888-93
Lemper, M; Leuckx, G; Heremans, Y et al. (2015) Reprogramming of human pancreatic exocrine cells to ?-like cells. Cell Death Differ 22:1117-30
Sasaki, Shugo; Miyatsuka, Takeshi; Matsuoka, Taka-aki et al. (2015) Activation of GLP-1 and gastrin signalling induces in vivo reprogramming of pancreatic exocrine cells into beta cells in mice. Diabetologia 58:2582-91
Ghosh, Rajarshi; Wang, Likun; Wang, Eric S et al. (2014) Allosteric inhibition of the IRE1? RNase preserves cell viability and function during endoplasmic reticulum stress. Cell 158:534-48
Miyatsuka, Takeshi; Matsuoka, Taka-aki; Sasaki, Shugo et al. (2014) Chronological analysis with fluorescent timer reveals unique features of newly generated ?-cells. Diabetes 63:3388-93
Baeyens, Luc; Lemper, Marie; Leuckx, Gunter et al. (2014) Transient cytokine treatment induces acinar cell reprogramming and regenerates functional beta cell mass in diabetic mice. Nat Biotechnol 32:76-83
Chamberlain, Chester E; Scheel, David W; McGlynn, Kathleen et al. (2014) Menin determines K-RAS proliferative outputs in endocrine cells. J Clin Invest 124:4093-101
German, Michael S (2013) Anonymous sources: where do adult ? cells come from? J Clin Invest 123:1936-8

Showing the most recent 10 out of 69 publications