This project previously identified the membrane scavenger receptor CD36 as a facilitator of cellular uptake of long chain fatty acid (FA) and showed that its deletion in mice markedly reduces FA uptake by heart, skeletal muscle and adipose tissue. The deletion also reduces gut chylomicron secretion and delays clearance of postprandial lipids. This renewal submission is focused on CD36-dependent FA signaling. CD36 can transduce intracellular signals through its interaction with tyrosine kinases and our recent findings support importance of this signaling in determining the metabolic fate of the FA. FA-induced CD36 signaling triggers conversion of polyunsaturated FA into the bioactive eicosanoids, mediates neurotransmitter release for gustatory fat perception, directs absorbed FA to chylomicron formation and triggers release by enteroendocrine cells of cholecystokinin and secretin, peptides with important roles in fat absorption and satiety. Our preliminary data also show that in myocytes, FA uptake enhances FA oxidation via CD36 signaling to activate the LKB1-AMPK pathway. Importance of CD36 to human FA metabolism was demonstrated by several groups including ours and genetic variants in CD36 were shown to associate with risk of the metabolic syndrome. Our recent data on the relationships between tissue expression profiles of CD36 transcripts and insulin sensitivity of obese subjects strongly support a key role for adipocyte CD36, which was a significant predictor of insulin sensitivity systemically and in liver and muscle.
In aim 1, we will determine the metabolic and disease significance of CD36-mediated FA signaling using in vitro cell systems and mice models.
In aim 2, we propose based on our recent identification of the CD36 amino acid residue required for FA uptake and signaling to examine the structural properties of the FA binding pocket and the influence of FA binding on CD36 membrane configuration. The CD36-protein interactions important for signal transduction will also be examined.
In aim 3, to gain insight into tissue specific regulation of CD36 level which determines FA homeostasis across tissues we will explore the factors that regulate alternate promoter usage of the human CD36 gene and the stability of alternate transcripts. The studies proposed should provide information on the importance of FA signaling in the regulation of FA utilization and metabolic health. The insight generated into CD36 structure and the regulation of tissue CD36 expression could help design strategies to target the protein more specifically and in a tissue specific manner.

Public Health Relevance

CD36 is a key metabolic protein in humans that functions in cellular fatty acid (FA) uptake and intracellular signaling and has been linked to the risk of the metabolic syndrome. Our recent data support importance of CD36 signaling in FA homeostasis and of adipocyte CD36 in systemic and tissue (liver, muscle) insulin sensitivity. The work proposed will provide insight into CD36 function, its structure in the membrane and the tissue specific regulation of its expression. It could help design more specific strategies to target CD36 in a tissue specific manner.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
2R01DK033301-28
Application #
8697642
Study Section
Clinical, Integrative and Molecular Gastroenterology Study Section (CIMG)
Program Officer
Pawlyk, Aaron
Project Start
1983-12-01
Project End
2018-03-31
Budget Start
2014-04-08
Budget End
2015-03-31
Support Year
28
Fiscal Year
2014
Total Cost
$339,034
Indirect Cost
$116,534
Name
Washington University
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
068552207
City
Saint Louis
State
MO
Country
United States
Zip Code
63130
Xie, Yan; Cifarelli, Vincenza; Pietka, Terri et al. (2017) Cd36 knockout mice are protected against lithogenic diet-induced gallstones. J Lipid Res 58:1692-1701
Scerbo, Diego; Son, Ni-Huiping; Sirwi, Alaa et al. (2017) Kidney triglyceride accumulation in the fasted mouse is dependent upon serum free fatty acids. J Lipid Res 58:1132-1142
Cifarelli, Vincenza; Ivanov, Stoyan; Xie, Yan et al. (2017) CD36 deficiency impairs the small intestinal barrier and induces subclinical inflammation in mice. Cell Mol Gastroenterol Hepatol 3:82-98
Shibao, Cyndya A; Celedonio, Jorge E; Ramirez, Claudia E et al. (2016) A Common CD36 Variant Influences Endothelial Function and Response to Treatment with Phosphodiesterase 5 Inhibition. J Clin Endocrinol Metab 101:2751-8
Hu, Xiaoqian; Cifarelli, Vincenza; Sun, Shishuo et al. (2016) Major role of adipocyte prostaglandin E2 in lipolysis-induced macrophage recruitment. J Lipid Res 57:663-73
Love-Gregory, Latisha; Kraja, Aldi T; Allum, Fiona et al. (2016) Higher chylomicron remnants and LDL particle numbers associate with CD36 SNPs and DNA methylation sites that reduce CD36. J Lipid Res 57:2176-2184
Abumrad, Nada A; Goldberg, Ira J (2016) CD36 actions in the heart: Lipids, calcium, inflammation, repair and more? Biochim Biophys Acta 1861:1442-9
Chami, Nathalie; Chen, Ming-Huei; Slater, Andrew J et al. (2016) Exome Genotyping Identifies Pleiotropic Variants Associated with Red Blood Cell Traits. Am J Hum Genet 99:8-21
Sundaresan, Sinju; Abumrad, Nada A (2015) Dietary Lipids Inform the Gut and Brain about Meal Arrival via CD36-Mediated Signal Transduction. J Nutr 145:2195-200
Harris, Lydia-Ann L S; Skinner, James R; Shew, Trevor M et al. (2015) Perilipin 5-Driven Lipid Droplet Accumulation in Skeletal Muscle Stimulates the Expression of Fibroblast Growth Factor 21. Diabetes 64:2757-68

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