Studies on mechanisms of action of hormones are performed in the least ambiguous fashion when the environment of the target cell can be closely controlled, and when cause-and-effect (not simply correlative) relationships can be established. Previous work in this laboratory on hamster yolk-sac erythroid cells has shown that these cells: undergo nearly the entire spectrum of globin ontogeny in the embryonic circulation; demonstrate globin ontogeny when grown in culture if both erythropoietin and triiodothyronine are present in the culture medium; and possess plasma-membrane receptors specific for erythropoietin and nuclear receptors specific for triiodothyronine. We now propose experiments designed to investigate responses of hamster yolk-sac erythroid cells to these hormones, singly and together. The experimental approach will include attempts to isolate the hormone-specific receptors and attempts to quantify receptor- specific mRNA.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK039751-03
Application #
3239691
Study Section
Diabetes and Digestive and Kidney Diseases Special Grants Review Committee (DDK)
Project Start
1987-09-30
Project End
1990-08-31
Budget Start
1989-09-01
Budget End
1990-08-31
Support Year
3
Fiscal Year
1989
Total Cost
Indirect Cost
Name
St. Luke's-Roosevelt Institute for Health Science
Department
Type
DUNS #
City
New York
State
NY
Country
United States
Zip Code
10019
Boussios, T; Lee, K M; Li, H et al. (1993) Expression of adult globin mRNAs in hamster yolk-sac erythroid cells during ontogeny. Exp Hematol 21:901-6
Li, H; Subar, M; Lee, K M et al. (1992) Cloning and sequence analysis of two embryonic beta-like globin cDNAs (y and z) of hamster. Biochim Biophys Acta 1130:218-20
Lee, K M; Subar, M; Li, H et al. (1992) Cloning of two adult hamster globin cDNAs (alpha and beta major). Biochim Biophys Acta 1130:343-4
Lee, K M; Bertles, J F; Boussios, T (1991) Translational control of globin chain ontogeny in hamster yolk sac erythroid cells. J Biol Chem 266:20555-60
Boussios, T; Bertles, J F; Goldwasser, E (1989) Erythropoietin. Receptor characteristics during the ontogeny of hamster yolk sac erythroid cells. J Biol Chem 264:16017-21