Psoriasis, asthma and inflammatory bowel disease (IBD) are characterized by a primary neutrophil and eosinophil infiltration at the inflammatory sites. 5-Oxo-ETE (5-oxo-6E,8Z,11Z,14Z-eicosatetraenoic acid) is a potent activator of both neutrophils and eosinophils and among lipid mediators; it is the most active eosinophil chemoattractant so far tested. This raises the possibility that this compound is an important mediator of inflammation, especially in conditions associated with eosinophilia. The major focus of this application is the development of a variety of tools that will be used to investigate the biosynthesis, metabolism and physiological role of 5-oxo-ETE. A primary goal will be to synthesize affinity ligands and photoaffinity probes, based on 5-HETE, to permit the purification, labeling and ultimate cloning of 5-hydroxyeicosanoid dehydrogenase, the enzyme responsible for the formation of 5-oxo-ETE. Similar approaches will be used to develop affinity ligands related to 5-oxo-ETE. These will be used to characterize metabolic enzymes (5- oxo-eicosanoid-D6-reductase and 5-ketoreductase) as well as the 5-oxo-ETE receptor. We recently found that human platelets convert 5-oxo-ETE to a metabolite (5-oxo-12S-HETE) that antagonizes 5-oxo-ETE-induced calcium mobilization, and we propose to use this as a lead compound to develop more potent antagonists. Another major or goal is to investigate the metabolism of other oxo-eicosanoids, including 12-oxo-LTB4. We found that this compound is converted by keratinocytes in a highly stereospecific manner to a series of cysteinyl-containing metabolites c-LTB3, d-LTB3 and e-LTB3, similar in structure to the potent cysteinyl-leukotrienes LTC4 and LTD4 and we propose to further investigate the mechanism for the formation of these compounds. In addition, we will investigate reductive pathways responsible for the conversion of 12-oxo-ETE to the potent proinflammatory agent 12-HETrE (12R-hydroxy-5Z,8Z,14Z-eicosatrienoic acid).

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK044730-10
Application #
6624870
Study Section
Bio-Organic and Natural Products Chemistry Study Section (BNP)
Program Officer
Tondravi, Mehrdad M
Project Start
1992-09-30
Project End
2004-11-30
Budget Start
2002-12-01
Budget End
2003-11-30
Support Year
10
Fiscal Year
2003
Total Cost
$244,640
Indirect Cost
Name
Florida Institute of Technology
Department
Type
Organized Research Units
DUNS #
053396669
City
Melbourne
State
FL
Country
United States
Zip Code
32901
Jacobo, Sheila H; Chang, Chih-Tsung; Lee, Gue-Jae et al. (2006) Total synthesis of 8,12-iso-iPF3alpha-VI, an EPA-derived isoprostane: stereoselective introduction of the fifth asymmetric center. J Org Chem 71:1370-9
Powell, William S; Rokach, Joshua (2005) Biochemistry, biology and chemistry of the 5-lipoxygenase product 5-oxo-ETE. Prog Lipid Res 44:154-83
Kim, Seongjin; Bellone, Sophie; Maxey, Kirk M et al. (2005) Synthesis of 15R-PGD2: a potential DP2 receptor agonist. Bioorg Med Chem Lett 15:1873-6
Monneret, Guillaume; Boumiza, Radhia; Gravel, Sylvie et al. (2005) Effects of prostaglandin D(2) and 5-lipoxygenase products on the expression of CD203c and CD11b by basophils. J Pharmacol Exp Ther 312:627-34
Kim, Seongjin; Powell, William S; Lawson, John A et al. (2005) iPF2alpha-III-17,18,19,20-d4: total synthesis and metabolism. Bioorg Med Chem Lett 15:1613-7
Mercier, Frederic; Morin, Caroline; Cloutier, Martin et al. (2004) 5-Oxo-ETE regulates tone of guinea pig airway smooth muscle via activation of Ca2+ pools and Rho-kinase pathway. Am J Physiol Lung Cell Mol Physiol 287:L631-40
Rokach, Joshua; Kim, Seongjin; Bellone, Sophie et al. (2004) Total synthesis of isoprostanes: discovery and quantitation in biological systems. Chem Phys Lipids 128:35-56
Stamatiou, Panagiota B; Chan, Chi-Chung; Monneret, Guillaume et al. (2004) 5-oxo-6,8,11,14-eicosatetraenoic acid stimulates the release of the eosinophil survival factor granulocyte/macrophage colony-stimulating factor from monocytes. J Biol Chem 279:28159-64
Pratico, Domenico; Rokach, Joshua; Lawson, John et al. (2004) F2-isoprostanes as indices of lipid peroxidation in inflammatory diseases. Chem Phys Lipids 128:165-71
Erlemann, Karl-Rudolf; Rokach, Joshua; Powell, William S (2004) Oxidative stress stimulates the synthesis of the eosinophil chemoattractant 5-oxo-6,8,11,14-eicosatetraenoic acid by inflammatory cells. J Biol Chem 279:40376-84

Showing the most recent 10 out of 40 publications