Abstract

The gastric enterochromaffin-like (ECL) cell is the cell responsible for histamine release within the gastric fundic mucosa, deemed essential for activation of the H2 receptor on the parietal cell. It therefore plays a central role in regulation of acid secretion. It constitutes 1% of cell volume in fundic epithelium. It has been isolated and purified to 85% cell content from rat gastric mucosa, by a combination of elutriation, density gradient centrifugation and short term culture. Histamine release is stimulated by gastrin/CCK, carbachol and epinephrine and inhibited by somatostatin, H3 receptor agonist and CCK B antagonists. The mechanisms of stimulus secretion coupling in this cell are unknown. These will be investigated by image analysis of changes in intracellular calcium, chloride and pH, by cell attached and whole cell measurements and by defining the proteins involved in granule exocytosis by screening a cDNA library derived from the purified ECL-cell preparation. These data will allow a novel understanding of the peripheral regulation of gastric acid secretion as well as the exocytotic process in a non- excitable endocrine cell.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
3R01DK046917-04S1
Application #
6022085
Study Section
General Medicine A Subcommittee 2 (GMA)
Program Officer
Hamilton, Frank A
Project Start
1994-05-01
Project End
1999-04-30
Budget Start
1997-05-01
Budget End
1999-04-30
Support Year
4
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
University of California Los Angeles
Department
Physiology
Type
Schools of Medicine
DUNS #
119132785
City
Los Angeles
State
CA
Country
United States
Zip Code
90095

  Publications

Sachs, G; Marcus, E A; Wen, Y et al. (2018) Editorial: control of acid secretion. Aliment Pharmacol Ther 48:682-683
Wen, Yi; Scott, David R; Vagin, Olga et al. (2018) Measurement of Internal pH in Helicobacter pylori by Using Green Fluorescent Protein Fluorimetry. J Bacteriol 200:
Wen, Yi; Feng, Jing; Sachs, George (2013) Helicobacter pylori 5'ureB-sRNA, a cis-encoded antisense small RNA, negatively regulates ureAB expression by transcription termination. J Bacteriol 195:444-52
Wen, Yi; Feng, Jing; Scott, David R et al. (2011) A cis-encoded antisense small RNA regulated by the HP0165-HP0166 two-component system controls expression of ureB in Helicobacter pylori. J Bacteriol 193:40-51
Wen, Yi; Feng, Jing; Scott, David R et al. (2009) The pH-responsive regulon of HP0244 (FlgS), the cytoplasmic histidine kinase of Helicobacter pylori. J Bacteriol 191:449-60
Vagin, Olga; Tokhtaeva, Elmira; Yakubov, Iskandar et al. (2008) Inverse correlation between the extent of N-glycan branching and intercellular adhesion in epithelia. Contribution of the Na,K-ATPase beta1 subunit. J Biol Chem 283:2192-202
Sachs, George; Shin, Jai Moo; Vagin, Olga et al. (2007) The gastric H,K ATPase as a drug target: past, present, and future. J Clin Gastroenterol 41 Suppl 2:S226-42
Munson, Keith; Law, Richard J; Sachs, George (2007) Analysis of the gastric H,K ATPase for ion pathways and inhibitor binding sites. Biochemistry 46:5398-417
Wen, Yi; Feng, Jing; Scott, David R et al. (2007) The HP0165-HP0166 two-component system (ArsRS) regulates acid-induced expression of HP1186 alpha-carbonic anhydrase in Helicobacter pylori by activating the pH-dependent promoter. J Bacteriol 189:2426-34
Zer, Cindy; Sachs, George; Shin, Jai Moo (2007) Identification of genomic targets downstream of p38 mitogen-activated protein kinase pathway mediating tumor necrosis factor-alpha signaling. Physiol Genomics 31:343-51

Showing the most recent 10 out of 58 publications