Abstract

This proposal is for work to further develop and characterize three dielectrophoretic cell separators for use with T-lymphocytes, B-leukocytes, monocytes, and granulocytes. The dielectrophoretic cell separation approach depends on alternating current (AC) electrokinetic phenomena which induce forces on cells that are dependent on cell composition, cell surface geometry. Three separator strategies will be explored: i) based on dielectric affinity, ii) dielectrophoresis combined with field flow fractionation, and iii) two dimensional dielectrophoresis in a traveling wave configuration.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK051065-03
Application #
2701200
Study Section
Special Emphasis Panel (ZRG7-SSS-3 (22))
Project Start
1996-05-01
Project End
1999-07-31
Budget Start
1998-05-01
Budget End
1999-07-31
Support Year
3
Fiscal Year
1998
Total Cost
Indirect Cost

  Institution

Name
University of Texas MD Anderson Cancer Center
Department
Pathology
Type
Other Domestic Higher Education
DUNS #
001910777
City
Houston
State
TX
Country
United States
Zip Code
77030

  Publications

Wang, Xujing; Becker, Frederick F; Gascoyne, Peter R C (2010) The fractal dimension of cell membrane correlates with its capacitance: a new fractal single-shell model. Chaos 20:043133
Das, Chandra M; Becker, Frederick; Vernon, Suzanne et al. (2005) Dielectrophoretic segregation of different human cell types on microscope slides. Anal Chem 77:2708-19
Wang, Xujing; Becker, Frederick F; Gascoyne, Peter R C (2002) Membrane dielectric changes indicate induced apoptosis in HL-60 cells more sensitively than surface phosphatidylserine expression or DNA fragmentation. Biochim Biophys Acta 1564:412-20
Gascoyne, Peter; Mahidol, Chulabhorn; Ruchirawat, Mahidol et al. (2002) Microsample preparation by dielectrophoresis: isolation of malaria. Lab Chip 2:70-5
Yang, J; Huang, Y; Wang, X B et al. (2000) Differential analysis of human leukocytes by dielectrophoretic field-flow-fractionation. Biophys J 78:2680-9
Wang, X B; Yang, J; Huang, Y et al. (2000) Cell separation by dielectrophoretic field-flow-fractionation. Anal Chem 72:832-9
Yang, J; Huang, Y; Wang, X B et al. (1999) Cell separation on microfabricated electrodes using dielectrophoretic/gravitational field-flow fractionation. Anal Chem 71:911-8
Wang, X; Yang, J; Gascoyne, P R (1999) Role of peroxide in AC electrical field exposure effects on friend murine erythroleukemia cells during dielectrophoretic manipulations. Biochim Biophys Acta 1426:53-68
Yang, J; Huang, Y; Wang, X et al. (1999) Dielectric properties of human leukocyte subpopulations determined by electrorotation as a cell separation criterion. Biophys J 76:3307-14
Huang, Y; Wang, X B; Gascoyne, P R et al. (1999) Membrane dielectric responses of human T-lymphocytes following mitogenic stimulation. Biochim Biophys Acta 1417:51-62

Showing the most recent 10 out of 14 publications