This is the second renewal application for this project to understand the role of genetic changes in the pathogenesis of glomerular diseases like focal segmental glomerulosclerosis (FSGS). In this application, we propose two specific aims. In the first, we propose to characterize a gene that we discovered that is highly expressed in podocytes. This gene, ARHGAP24, is a known regulator of the actin cytoskeleton and the high expression of this gene implicates a specific actin regulatory pathway in the normal function of podocytes. To test the role of this gene in the glomerulus, we propose to generate and characterize a mouse that lacks expression of ARHGAP24. In the second aim, we will set-up a genetic screen that combines RNAi technology and state of the art mouse genetic methods to perform a genetic screen in mouse to identify genes that when mutated contribute with CD2AP and another podocyte specific gene, synaptopodin, in the pathogenesis of glomerular dysfunction. Our long-term goal is the identification of all genes that, when mutated, contribute to the pathogenesis of human FSGS.

Public Health Relevance

The goals of this project are to better understand the genetic causes of kidney disease. We will test the role of specific genes in the development of kidney diseases.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK058366-12
Application #
8144356
Study Section
Pathobiology of Kidney Disease Study Section (PBKD)
Program Officer
Mullins, Christopher V
Project Start
2000-09-15
Project End
2015-06-30
Budget Start
2011-07-01
Budget End
2012-06-30
Support Year
12
Fiscal Year
2011
Total Cost
$270,631
Indirect Cost
Name
Washington University
Department
Pathology
Type
Schools of Medicine
DUNS #
068552207
City
Saint Louis
State
MO
Country
United States
Zip Code
63130
Suleiman, Hani Y; Roth, Robyn; Jain, Sanjay et al. (2017) Injury-induced actin cytoskeleton reorganization in podocytes revealed by super-resolution microscopy. JCI Insight 2:
Beckerman, Pazit; Bi-Karchin, Jing; Park, Ae Seo Deok et al. (2017) Transgenic expression of human APOL1 risk variants in podocytes induces kidney disease in mice. Nat Med 23:429-438
Malone, Andrew F; Funk, Steven D; Alhamad, Tarek et al. (2017) Functional assessment of a novel COL4A5 splice region variant and immunostaining of plucked hair follicles as an alternative method of diagnosis in X-linked Alport syndrome. Pediatr Nephrol 32:997-1003
Tsuji, Kenji; Suleiman, Hani; Miner, Jeffrey H et al. (2017) Ultrastructural Characterization of the Glomerulopathy in Alport Mice by Helium Ion Scanning Microscopy (HIM). Sci Rep 7:11696
Tsuji, Kenji; P?unescu, Teodor G; Suleiman, Hani et al. (2017) Re-characterization of the Glomerulopathy in CD2AP Deficient Mice by High-Resolution Helium Ion Scanning Microscopy. Sci Rep 7:8321
Bartlett, Christina S; Scott, Rizaldy P; Carota, Isabel Anna et al. (2017) Glomerular mesangial cell recruitment and function require the co-receptor neuropilin-1. Am J Physiol Renal Physiol 313:F1232-F1242
Kim, Alfred Hj; Chung, Jun-Jae; Akilesh, Shreeram et al. (2017) B cell-derived IL-4 acts on podocytes to induce proteinuria and foot process effacement. JCI Insight 2:
Randles, Michael J; Collinson, Sophie; Starborg, Tobias et al. (2016) Three-dimensional electron microscopy reveals the evolution of glomerular barrier injury. Sci Rep 6:35068
Grahammer, Florian; Wigge, Christoph; Schell, Christoph et al. (2016) A flexible, multilayered protein scaffold maintains the slit in between glomerular podocytes. JCI Insight 1:
Brähler, Sebastian; Yu, Haiyang; Suleiman, Hani et al. (2016) Intravital and Kidney Slice Imaging of Podocyte Membrane Dynamics. J Am Soc Nephrol 27:3285-3290

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