Primary hyperparathyroidism is a common human endocrinopathy, and cyclin D1 (PRAD1) oncogene overexpression contributes to the pathogenesis of up to 40% of parathyroid adenomas. Numerous studies have led to the widely accepted paradigm that cyclin D1 functions in a biochemical pathway with cyclin- dependent kinase (cdk) 4 or 6, which it activates, and the retinoblastoma oncosuppressor protein pRB, considered the primary substrate of cyclin D1-activated cdk. These molecules have tremendous significance to neoplasia, being targeted by mutation or regulatory derangements in most or perhaps even all cancers. These concepts, however, may not fully reflect cyclin D1's true role when overexpressed in the complex in vivo process of tumorigenesis, and recent data indeed suggest other, non-cdk-dependent roles for cyclin D1. Such alternative mechanisms might be especially important in parathyroid tumors, possibly explaining why these cyclin D1-driven neoplasms are almost always benign in marked contrast with the malignant features of other cyclin D1-induced tumors, and may be relevant to the mechanism through which cyclin D1 leads to dysregulation of the serum calcium parathyroid hormone setpoint. Further, cyclin D1-induced parathyroid tumorigenesis may be modulated by key regulators, most notably vitamin D status, but direct in vivo experimental evidence is lacking. Hyperparathyroid-prone transgenic mice with parathyroid-targeted overexpression of cyclin D1 constitute an especially relevant system in which to test these hypotheses. The proposed studies are designed to address the mechanisms through which cyclin D1, directly and as modified by vitamin D status, contributes to parathyroid neoplasia. The studies will (a) examine whether overexpressed cyclin D1 may contribute to parathyroid tumorigenesis through mechanisms apart from activation of cdk4/6, by developing and characterizing transgenic mice bearing a parathyroid-targeted mutant cyclin D1 transgene unable to activate cdk4 or 6, and (b) examine whether vitamin D deficiency directly alters the growth of cyclin D1-induced parathyroid tumors and modulates the phenotype of primary hyperparathyroidism, by imposing nutritional vitamin D deficiency upon transgenic mice with parathyroid-targeted overexpression of cyclin D1. These results will carry important implications to the molecular pathogenesis of parathyroid neoplasia, are potentially relevant to a variety of cyclin D1-driven cancers, and may ultimately contribute to novel treatment strategies. This research will uncover important molecular mechanisms causing hyperparathyroidism, a common human endocrine disorder in which osteoporosis occurs, and will likely have strong relevance to a variety of malignancies also caused by the cyclin D1 cancer gene, such as breast cancer, B-cell lymphoma, myeloma, and squamous cell cancers. Further, the results may ultimately contribute to novel treatment strategies for these diseases. Finally, vitamin D nutritional status, a major problem in aging Americans and also rampant in underdeveloped nations, is considered an important potential influence on the vigor of parathyroid (and other forms of) tumorigenicity, and our model system provides unique advantages in which this hypothesis can be tested.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK066411-05
Application #
8225313
Study Section
Skeletal Biology Development and Disease Study Section (SBDD)
Program Officer
Malozowski, Saul N
Project Start
2008-02-01
Project End
2015-01-31
Budget Start
2012-02-01
Budget End
2015-01-31
Support Year
5
Fiscal Year
2012
Total Cost
$308,241
Indirect Cost
$99,970
Name
University of Connecticut
Department
Other Basic Sciences
Type
Schools of Medicine
DUNS #
022254226
City
Farmington
State
CT
Country
United States
Zip Code
06030
Costa-Guda, Jessica; Arnold, Andrew (2014) Genetic and epigenetic changes in sporadic endocrine tumors: parathyroid tumors. Mol Cell Endocrinol 386:46-54
Costa-Guda, Jessica; Imanishi, Yasuo; Palanisamy, Nallasivam et al. (2013) Allelic imbalance in sporadic parathyroid carcinoma and evidence for its de novo origins. Endocrine 44:489-95