Chronic pancreatitis remains a common and challenging clinical syndrome. Its cardinal feature, pain, has been difficult to treat effectively despite a multitude of empirical therapeutic approaches. Our laboratory has been actively pursuing the molecular pathogenesis of pain in pancreatitis for over a decade. In the process we have established highly useful and relevant rodent models of CP and gained valuable insight into the contribution of specific molecules, particularly voltage dependent (Kv) and TRPV1 ion channels and nerve growth factor, NGF. However, it is clear that there is much more that we need to learn. In this proposal, we will focus on a previously under scribed role of transforming growth factor beta, (TGF?) in the pathogenesis of chronic inflammatory pain. Although TGF? is prominent in the inflammatory mileu, its participation in nociceptive sensitization has received little attention. Our hypothesis is that TGF? is an important modulator of sensory neuronal function and plays a major role in the pathogenesis of pain in chronic pancreatitis via changes in pancreas-specific sensory neuronal excitability and ion channel function. In this proposal, we will attempt to prove this hypothesis, using a comprehensive multidisciplinary approach encompassing molecular, electrophysiological and behavioral assays, via the following specific aims: (1) To determine the effects of TGF? on sensory neuronal excitability and ion channel activity in vitro (2) To determine the effects of exogenous TGF? on pain behavior and pancreatic sensory neuronal plasticity (in vivo) (3)To determine the role of endogenous TGF? in the pathogenesis of pain behavior and sensory neuronal plasticity in chronic pancreatitis. The significance of this proposal is two-fold. First, it will provide a better understanding of the pathogenesis of pain in chronic pancreatitis. Secondly, it will establish a major new biological role for TGF?, opening up the possibility of its involvement in many other chronic painful disorders that may be inflammatory or neoplastic in origin.

Public Health Relevance

This aim of this proposal is to understand the way in which inflammation in the pancreas causes pain and increases the activity of nerves that carry pain signals. Specifically, we are focusing on the role in this process of a molecule called transforming growth factor- beta, which has previously been implicated in the development of fibrosis.

National Institute of Health (NIH)
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Research Project (R01)
Project #
Application #
Study Section
Special Emphasis Panel (ZRG1-DKUS-C (03))
Program Officer
Serrano, Jose
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Johns Hopkins University
United States
Zip Code
Sinha, Smrita; Fu, Ya-Yuan; Grimont, Adrien et al. (2017) PanIN Neuroendocrine Cells Promote Tumorigenesis via Neuronal Cross-talk. Cancer Res 77:1868-1879
Sinha, Smrita; Fu, Ya-Yuan; Grimont, Adrien et al. (2017) PanIN neuroendocrine cells promote tumorigenesis via neuronal crosstalk. Cancer Res :
Moran, Robert A; James, Theodore; Pasricha, Pankaj Jay (2015) Pancreatic pain. Curr Opin Gastroenterol 31:407-15
Pasca di Magliano, Marina; Forsmark, Christopher; Freedman, Steven et al. (2013) Advances in acute and chronic pancreatitis: from development to inflammation and repair. Gastroenterology 144:e1-4
Pasricha, Pankaj Jay (2012) Unraveling the mystery of pain in chronic pancreatitis. Nat Rev Gastroenterol Hepatol 9:140-51
Zhu, Yaohui; Mehta, Kshama; Li, Cuiping et al. (2012) Systemic administration of anti-NGF increases A-type potassium currents and decreases pancreatic nociceptor excitability in a rat model of chronic pancreatitis. Am J Physiol Gastrointest Liver Physiol 302:G176-81
Zhu, Yaohui; Colak, Tugba; Shenoy, Mohan et al. (2012) Transforming growth factor beta induces sensory neuronal hyperexcitability, and contributes to pancreatic pain and hyperalgesia in rats with chronic pancreatitis. Mol Pain 8:65
Liu, LianSheng; Shenoy, Mohan; Pasricha, Pankaj Jay (2011) Substance P and calcitonin gene related peptide mediate pain in chronic pancreatitis and their expression is driven by nerve growth factor. JOP 12:389-94
Zhu, Yaohui; Colak, Tugba; Shenoy, Mohan et al. (2011) Nerve growth factor modulates TRPV1 expression and function and mediates pain in chronic pancreatitis. Gastroenterology 141:370-7
Hughes, Michael S; Shenoy, Mohan; Liu, Liansheng et al. (2011) Brain-derived neurotrophic factor is upregulated in rats with chronic pancreatitis and mediates pain behavior. Pancreas 40:551-6

Showing the most recent 10 out of 11 publications