Evidence from epidemiological studies indicates an inverse correlation between the incidence of certain immune-mediated diseases, including inflammatory bowel diseases (IBD), and exposure to parasitic helminths. Helminth infections are known to dampen Th1 reactions, a characteristic that has potential use in the treatment of Th1-mediated autoimmune and inflammatory conditions. However, our understanding of the exact mechanism by which helminths modulate mucosal responses is incomplete. Using our recently established co- infection model, we found that intestinal helminth infection exacerbates colitis induced by concurrent infection with the bacterial enteric pathogen Citrobacter rodentium. Additional preliminary results from studies using a T cell-mediated colitis model provide evidence to indicate that a simultaneous helminth infection also enhances intestinal inflammation in SCID mice that are adoptively transferred with ovalbumin (OVA) specific T cells and infected orally with OVA expressing E. coli. Helminth-induced exacerbation of intestinal inflammation has also been reported in an inducible colitis model. The differences in the effects of helminths in various models of intestinal inflammation emphasize the need for a better understanding of the mechanisms by which helminths modulate host mucosal immunity. Our observations of deleterious consequences of helminth parasites in both bacterial and T cell mediated intestinal inflammation support our overall hypothesis that helminth infection may be a potential risk factor for the development and progression of such diseases. Experiments proposed in this application address the idea that the exacerbating effect on intestinal inflammation is the consequence of helminth induced (1) modulation of T cell function to a phenotype that promotes pro-inflammatory responses, in part by impairing intestinal epithelial barrier function and allowing increased translocation of luminal contents, and (2) alterations in macrophage phenotype and function that compromise the ability to eliminate translocated bacteria. We will carry out experiments to explore the mechanism(s) by which enteric helminths modulate intestinal inflammatory responses. We will determine (1) how helminth infection alters the T cell response to a surrogate commensal bacterium (E. coli-OVA) and how helminth-induced dysregulation of T cell response impairs epithelial barrier function;and (2) the effect of helminths on macrophage microbicidal and inflammatory functions. The experiments proposed will provide new insight into the mechanism by which helminths modulate intestinal responses to luminal bacterial antigens, and facilitate the development of novel, safer and more effective treatments for immune-mediated diseases.
The studies outlined in this proposal will determine the immuomodulatory role of intestinal helminth parasites in bacteria-associated and T cell-mediated intestinal inflammation. We will carry out experiments to (1) explore the mechanism(s) by which enteric helminth alters the T cell response to the surrogate commensal bacterium (E. coli-OVA);and (2) determine how the helminth-induced Th2 response, in turn, impairs intestinal epithelial barrier function and alters macrophage phenotype and function that compromise the ability to eliminate translocated bacteria, contributing to impaired host defense and increased tissue injury.
|Su, Chien Wen; Chen, Chih-Yu; Li, Yali et al. (2018) Helminth infection protects against high fat diet-induced obesity via induction of alternatively activated macrophages. Sci Rep 8:4607|
|Su, C; Su, L; Li, Y et al. (2018) Helminth-induced alterations of the gut microbiota exacerbate bacterial colitis. Mucosal Immunol 11:144-157|
|Li, Yali; Lv, Meili; Su, Chienwen et al. (2017) p40 phox -Deficient Mice Exhibit Impaired Bacterial Clearance and Enhanced Pro-inflammatory Responses during Salmonella enterica serovar Typhimurium Infection. Front Immunol 8:1270|
|Trebicka, Estela; Shanmugam, Nanda Kumar N; Chen, Kejie et al. (2015) Intestinal Inflammation Leads to a Long-lasting Increase in Resistance to Systemic Salmonellosis that Requires Macrophages But Not B or T Lymphocytes at the Time of Pathogen Challenge. Inflamm Bowel Dis 21:2758-65|
|Meng, Di; Zhu, Weishu; Shi, Hai Ning et al. (2015) Toll-like receptor-4 in human and mouse colonic epithelium is developmentally regulated: a possible role in necrotizing enterocolitis. Pediatr Res 77:416-24|
|Weng, Meiqian; Ganguli, Kriston; Zhu, Weishu et al. (2014) Conditioned medium from Bifidobacteria infantis protects against Cronobacter sakazakii-induced intestinal inflammation in newborn mice. Am J Physiol Gastrointest Liver Physiol 306:G779-87|
|Su, Libo; Qi, Yujuan; Zhang, Mei et al. (2014) Development of fatal intestinal inflammation in MyD88 deficient mice co-infected with helminth and bacterial enteropathogens. PLoS Negl Trop Dis 8:e2987|
|Su, Libo; Su, Chien-wen; Qi, Yujuan et al. (2014) Coinfection with an intestinal helminth impairs host innate immunity against Salmonella enterica serovar Typhimurium and exacerbates intestinal inflammation in mice. Infect Immun 82:3855-66|
|Whary, Mark T; Muthupalani, Sureshkumar; Ge, Zhongming et al. (2014) Helminth co-infection in Helicobacter pylori infected INS-GAS mice attenuates gastric premalignant lesions of epithelial dysplasia and glandular atrophy and preserves colonization resistance of the stomach to lower bowel microbiota. Microbes Infect 16:345-55|
|Zhao, Y; Liu, M Y; Wang, X L et al. (2013) Modulation of inflammatory bowel disease in a mouse model following infection with Trichinella spiralis. Vet Parasitol 194:211-6|
Showing the most recent 10 out of 20 publications