Zebrafish has been widely used as a model animal to study vertebrate development and genetics. It is well established that genetic studies of zebrafish genes can provide insights into the function of the conserved vertebrate genes and therefore lead to better understanding of human diseases and health. With the zebrafish genome sequencing project nearly completed, it is highly desirable that all of the genes predicated by sequencing will also carry an mutation that readily available for gene function studies. Towards this goal, we have developed an efficient and streamlined platform using proviral insertions coupled with high-throughput sequencing and mapping technologies to mutagenize essentially all of the identifiable candidate genes in zebrafish genome. In this project we propose to provide genetic tools to the public research community by constructing a library of zebrafish strains in which approximately 12,000 zebrafish genes have been disrupted with a retrovirus insertion. All the resources generated will be made available to the community with a minimal cost before publication.

Public Health Relevance

Zebrafish is a popular vertebrate model organism for studying development and human diseases and we use state of the art technologies to generate mutations in most of zebrafish genes. Since these genes are highly conserved our studies will provide valuable information for understanding human diseases and assisting design of novel therapeutic approaches.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK084349-04
Application #
8325920
Study Section
Special Emphasis Panel (ZRG1-BDA-F (50))
Program Officer
Rasooly, Rebekah S
Project Start
2009-09-01
Project End
2014-08-31
Budget Start
2012-09-01
Budget End
2014-08-31
Support Year
4
Fiscal Year
2012
Total Cost
$328,285
Indirect Cost
$115,113
Name
University of California Los Angeles
Department
Biochemistry
Type
Schools of Arts and Sciences
DUNS #
092530369
City
Los Angeles
State
CA
Country
United States
Zip Code
90095
Yuan, Yike; Zhang, Yaguang; Yao, Shaohua et al. (2014) The translation initiation factor eIF3i up-regulates vascular endothelial growth factor A, accelerates cell proliferation, and promotes angiogenesis in embryonic development and tumorigenesis. J Biol Chem 289:28310-23
Varshney, Gaurav K; Lu, Jing; Gildea, Derek E et al. (2013) A large-scale zebrafish gene knockout resource for the genome-wide study of gene function. Genome Res 23:727-35
Zu, Yao; Tong, Xiangjun; Wang, Zhanxiang et al. (2013) TALEN-mediated precise genome modification by homologous recombination in zebrafish. Nat Methods 10:329-31
Lin, Sijie; Zhao, Yan; Nel, Andre E et al. (2013) Zebrafish: an in vivo model for nano EHS studies. Small 9:1608-18
Varshney, Gaurav K; Huang, Haigen; Zhang, Suiyuan et al. (2013) The Zebrafish Insertion Collection (ZInC): a web based, searchable collection of zebrafish mutations generated by DNA insertion. Nucleic Acids Res 41:D861-4