Our finding that AQP2 constitutively recycles in the absence of the antidiuretic hormone, vasopressin (VP), has led to a rethinking of how VP causes AQP2 membrane accumulation and urinary concentration. Indeed, we recently found that an FDA approved drug, simvastatin, blocks AQP2 endocytosis and reduces urine output in VP- deficient Brattleboro rats. Our proposed studies continue the strategy of combining cell biological interrogation of AQP2 trafficking with functional studies in vivo to generate novel therapeutic approaches for disorders of fluid balance including NDI, as well as congestive heart failure and hyponatremia.
In Aim 1, we will explore the mechanism(s) underlying our recent discoveries that AQP2 itself is a catalyst for VP-induced actin depolymerization and that the actin-binding protein ezrin is an AQP2 associated protein involved in AQP2 endocytosis. Agents that target the ezrin binding domain on AQP2 would provide increased specificity of action for NDI treatment.
Aim 2 will first expand our promising work on phosphodiesterase (PDE5) inhibitors, calcitonin and statins, which promote AQP2 membrane accumulation and increase urine concentration. We will optimize treatment doses, times and delivery routes, and assess the effectiveness of drug combinations. Then, we will further develop our new chemical screening program to identify and characterize compounds that modulate exocytosis and endocytosis in AQP2- expressing cells using high throughput, fluorescence-based assays in cell cultures (we have already identified 40 chemicals that need further characterization). Compounds that modify (increase or decrease) AQP2 membrane accumulation in vitro will ultimately be tested in vivo. We will use a multidisciplinary approach ranging from in vitro protei association assays, novel cell culture models, cellular imaging and analysis (using the PMB Microscopy Core), high throughout chemical screening (via the Center for Systems Biology Chemical Biology COre) and whole animal studies. Our overall goal is to design novel therapies for water balance disorders, including NDI and hyponatremia, using a combination of directed and discovery approaches that bypass the VP/V2R signaling cascade to modulate cell surface expression of AQP2.
The kidney controls how much of our daily water intake is reabsorbed back into the body, and how much is excreted in the urine. Sometimes this process is not balanced, resulting in diseases including hypertension (too much fluid and sodium reabsorption) or dehydration (too much urine production). Our work is aimed at finding the mechanisms by which the kidney controls these processes in order to cure kidney disease.
|Cheung, Pui W; Nomura, Naohiro; Nair, Anil V et al. (2016) EGF Receptor Inhibition by Erlotinib Increases Aquaporin 2-Mediated Renal Water Reabsorption. J Am Soc Nephrol 27:3105-3116|
|Cheung, Pui W; Nomura, Naohiro; Nair, Anil V et al. (2016) EGF Receptor Inhibition by Erlotinib Increases Aquaporin 2-Mediated Renal Water Reabsorption. J Am Soc Nephrol :|
|Nair, Anil V; Keliher, Edmund J; Core, Amanda B et al. (2015) Characterizing the interactions of organic nanoparticles with renal epithelial cells in vivo. ACS Nano 9:3641-53|
|Nunes, Paula; Roth, Isabelle; Meda, Paolo et al. (2015) Ionic imbalance, in addition to molecular crowding, abates cytoskeletal dynamics and vesicle motility during hypertonic stress. Proc Natl Acad Sci U S A 112:E3104-13|
|Rice, William L; Li, Wei; Mamuya, Fahmy et al. (2015) Polarized Trafficking of AQP2 Revealed in Three Dimensional Epithelial Culture. PLoS One 10:e0131719|
|Herak-Kramberger, Carol M; Breljak, Davorka; LjubojeviÄ‡, Marija et al. (2015) Sex-dependent expression of water channel AQP1 along the rat nephron. Am J Physiol Renal Physiol 308:F809-21|
|Arthur, Julian; Huang, Jianmin; Nomura, Naohiro et al. (2015) Characterization of the putative phosphorylation sites of the AQP2 C terminus and their role in AQP2 trafficking in LLC-PK1 cells. Am J Physiol Renal Physiol 309:F673-9|
|Nomura, Naohiro; Nunes, Paula; Bouley, Richard et al. (2014) High-throughput chemical screening identifies AG-490 as a stimulator of aquaporin 2 membrane expression and urine concentration. Am J Physiol Cell Physiol 307:C597-605|
|Nunes, Paula; Ernandez, Thomas; Roth, Isabelle et al. (2013) Hypertonic stress promotes autophagy and microtubule-dependent autophagosomal clusters. Autophagy 9:550-67|
|Bouley, Richard; Nunes, Paula; Andriopoulos Jr, Billy et al. (2013) Heterologous downregulation of vasopressin type 2 receptor is induced by transferrin. Am J Physiol Renal Physiol 304:F553-64|
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