A critical barrier to the identification of appropriate therapeutic targets to treat glomerular disease is the limited understanding of signaling cascades that regulate podocyte injury and renal survival. Our long term goal is to enhance the pipeline of putative therapeutic targets available to tackle human glomerular disease by elucidating the details and functional significance of key signaling pathways that regulate podocyte injury and survival. Our preliminary data have identified dendrin as a key pro injury signaling molecule that promotes podocyte depletion and is involved in podocyte mesangial cell crosstalk. We have identified human dendrin gene variants that segregate with familial steroid resistant focal segmental glomerulosclerosis (FSGS) and identified the pro survival Hippo pathway effector Yes associated protein (YAP) as an inhibitor of dendrin function. The overall objective of this application is to define the role of dendrin as a key mediator of pathogenic podocyte mesangial cell crosstalk and subsequent glomerular disease progression.Our central hypothesis is that dendrin is regulated in a phosphorylation dependent manner by YAP, where disease associated dendrin mutants may induce podocyte injury though decreased YAP inhibition. Dendrin regulated soluble factors from podocytes promote mesangial cell fibronectin secretion, leading to mesangial matrix expansion and glomerular disease progression. The rationale for the proposed research is that defining how dendrin function is inhibited by YAP and characterizing its role in glomerular disease progression will advance understanding of glomerular disease progression as well as the quest for novel therapeutic targets available for clinical use. Our hypothesis will be tested by pursuing two specific aims:
Aim 1 will explore the inhibition of wild type and FSGS associated dendrin mutants by YAP. We will define the molecular interaction between phosphorylated YAP and the dendrin isoforms and characterize the consequences of binding in the context of cell survival and dendrin subcellular localization and function. We will also test in vivo whether transgenic expression of YAP in podocytes slows murine glomerular disease progression by inhibiting dendrin function.
In Aim 2 we will define the role of dendrin in pathogenic podocyte mesangial cell crosstalk using our novel established in vitro cell systems as well as an inducible transgenic mouse model of crosstalk. Our innovative approach utilizes state of the art proteomics technology to identify dendrin regulated soluble factors from podocytes that induce mesangial cell fibronectin secretion. These contributions are significant because they represent the first step in a continuum of research that is expected to advance understanding of glomerular disease progression and identify therapeutic targets and strategies to improve clinical outcomes.

Public Health Relevance

Our preliminary data identified the proinjury signaling molecule dendrin as a key mediator of progressive glomerular disease, a group of clinical disorders whose pathogenesis is not well understood and for which there are limited and nonspecific treatment options. The proposed research will advance the currently available knowledge of glomerular disease pathogenesis and serve as a platform for the development of novel therapeutic agents.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
1R01DK103022-01A1
Application #
8963906
Study Section
Pathobiology of Kidney Disease Study Section (PBKD)
Program Officer
Rys-Sikora, Krystyna E
Project Start
2015-09-10
Project End
2020-07-31
Budget Start
2015-09-10
Budget End
2016-07-31
Support Year
1
Fiscal Year
2015
Total Cost
$381,375
Indirect Cost
$156,375
Name
Icahn School of Medicine at Mount Sinai
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
078861598
City
New York
State
NY
Country
United States
Zip Code
10029
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Ashraf, Shazia; Kudo, Hiroki; Rao, Jia et al. (2018) Mutations in six nephrosis genes delineate a pathogenic pathway amenable to treatment. Nat Commun 9:1960
Meliambro, Kristin; Wong, Jenny S; Ray, Justina et al. (2017) The Hippo pathway regulator KIBRA promotes podocyte injury by inhibiting YAP signaling and disrupting actin cytoskeletal dynamics. J Biol Chem 292:21137-21148
Angeletti, Andrea; Reyes-Bahamonde, Joselyn; Cravedi, Paolo et al. (2017) Complement in Non-Antibody-Mediated Kidney Diseases. Front Med (Lausanne) 4:99
Shirata, Naritoshi; Ihara, Kan-Ichiro; Yamamoto-Nonaka, Kanae et al. (2017) Glomerulosclerosis Induced by Deficiency of Membrane-Associated Guanylate Kinase Inverted 2 in Kidney Podocytes. J Am Soc Nephrol 28:2654-2669
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Cravedi, Paolo; Campbell, Kirk N (2016) ACTH action on podocytes: mystery solved? Am J Physiol Renal Physiol 310:F1178-9
Wong, Jenny S; Meliambro, Kristin; Ray, Justina et al. (2016) Hippo signaling in the kidney: the good and the bad. Am J Physiol Renal Physiol 311:F241-8
Schwartzman, Monica; Reginensi, Antoine; Wong, Jenny S et al. (2016) Podocyte-Specific Deletion of Yes-Associated Protein Causes FSGS and Progressive Renal Failure. J Am Soc Nephrol 27:216-26

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