Chronic pancreatitis (CP) is characterized by inflammation, fibrosis, and loss of pancreatic cells. CP can lead to sufficient tissue destruction and result in exocrine and endocrine insufficiency as well as difficult to treat chronic abdominal pain. The management of CP is challenging as there are no effective methods that can stop progression or reverse the disease. In addition, CP is a risk factor for the development of pancreatic cancer. Absence of progress in CP therapy in part is due to lack of understanding in mechanisms that potentially can either reverse or halt the disease. Recent in vitro and in vivo studies have shown objectively the role of activated pancreatic stellate cells (PSCs) in fibrogenesis in CP. PSCs play a central role in disease progression by regulating the synthesis and degradation of extracellular matrix proteins. Activation of PSCs is increased by cytokines from injured acinar cell and infiltrating leukocytes (e.g. macrophages). However, the mechanisms by which macrophages trigger and sustain the fibrotic processes are not fully understood. Our initial studies show that subset of macrophages (known as M2) are dominant in CP as compared to acute pancreatitis (AP), which is dominated by a different subtype of macrophages (M1). We hypothesize that unlike M1, M2 macrophages promote pancreatic fibrosis through sustained activation of PSCs, and in turn the activated PSCs enhance macrophage polarization towards M2 and propose mechanisms involved in macrophage-PSCs interaction to promote progression of CP. To achieve this goal in aim 1, we use animal models of acute and chronic pancreatitis in order to characterize immune responses associated with CP.
Under aim 2, we will determine the role and source of macrophage subpopulation in CP. Specifically, we propose to test and study subtypes of macrophages and cytokines that are essential in the development of CP.
Under aim 3, we will identify mechanisms via which macrophages affect PSC function and how PSCs in turn affect macrophage polarization and behavior using various co-culture systems and in vivo using transgenic animals that allow testing of pertinent pathways in the immune responses. When possible human PSCs (isolated from surgical specimens) and monocyte/macrophages will be used to confirm and identify pathways involved. Our proposed studies are novel because they focus on the immune responses associated with CP, macrophage plasticity, and have the potential to shift paradigm in the field by proposing mechanisms that can either halt or reverse CP, and thus alter the natural course of the disease. In addition to the gained understanding of immune mechanisms that mediate and/or allow progression of CP, the potential impact of this project is of great clinical significance, as our studies may lead to development of novel therapies that can change how we manage patients with CP.

Public Health Relevance

Chronic pancreatitis (CP) remains a challenging clinical problem and despite its disease burden, therapy remains supportive at best. Generally chronic pancreatitis is accepted as an irreversible fibrotic disease and current chronic pancreatitis management focus in dealing with the complications associated with the disease, as there is no active therapy for the condition that would alter the natural course of the disease. Our study proposes to examine the immune responses associated with progression and/or development of chronic pancreatitis in order to better understand pathogenic mechanisms that lead to disease progression. Using experimental models of chronic pancreatitis, we take advantage of the plasticity of immune cells and associated immune responses to interfere with chronic pancreatitis progression pathways. Our preliminary results identify some of the important immune mechanisms involved and highlight the possibility of interfering with these immune pathways to confer protection against chronic pancreatitis.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK105263-02
Application #
9253388
Study Section
Special Emphasis Panel (ZRG1)
Program Officer
Serrano, Jose
Project Start
2016-04-05
Project End
2021-03-31
Budget Start
2017-04-01
Budget End
2018-03-31
Support Year
2
Fiscal Year
2017
Total Cost
Indirect Cost
Name
Stanford University
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
009214214
City
Stanford
State
CA
Country
United States
Zip Code
94304
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Gukovskaya, Anna S; Gukovsky, Ilya; Alg├╝l, Hana et al. (2017) Autophagy, Inflammation, and Immune Dysfunction in the Pathogenesis of Pancreatitis. Gastroenterology 153:1212-1226
Xue, Jing; Zhao, Qinglan; Sharma, Vishal et al. (2016) Aryl Hydrocarbon Receptor Ligands in Cigarette Smoke Induce Production of Interleukin-22 to Promote Pancreatic Fibrosis in Models of Chronic Pancreatitis. Gastroenterology 151:1206-1217
Habtezion, Aida (2015) Inflammation in acute and chronic pancreatitis. Curr Opin Gastroenterol 31:395-9