? The long term objective of our research is to develop new compositions and technologies for controlling biomolecular recognition processes in the rapidly developing fields of micro- and nano-scale sensing and diagnostic devices. In the first eight years of this research program, we have developed a new approach to the control of biomolecular recognition that is based on coupling the stimulated collapse (dehydration) or expansion (rehydration) of the """"""""smart"""""""" polymer coil with recognition events such as protein-ligand, enzyme-substrate, protein-protein and DNA hybridization reactions. In this approach, the smart polymers serve as both antennae and actuators, to sense signals and respond to them, leading to control of biorecognition events. Their characteristic sharp responses in coil size and physical properties to small changes in pH, temperature, and/or electromagnetic irradiation over narrow ranges or at specific wavelengths permits rapid and precise control of molecular events. We term this a """"""""molecular switch"""""""". We seek here to continue the development and applications of such molecular switches with affinity proteins, ligands and enzymes, as well as to develop new strategies for releasing captured targets on command, and for """"""""molecular matchmaking"""""""" that selectively and reversibly brings together specific, interactive molecular components in complex mixtures. The molecular engineering underlying these aims spans sophisticated polymer design and synthesis and protein and DNA engineering, and matches the functional properties of the stimuli-responsive polymers to the molecular complexities of ligand binding pockets, active sites, and receptor-ligand sequences. ? ?

Agency
National Institute of Health (NIH)
Institute
National Institute of Biomedical Imaging and Bioengineering (NIBIB)
Type
Research Project (R01)
Project #
5R01EB000252-09
Application #
6711696
Study Section
Surgery and Bioengineering Study Section (SB)
Program Officer
Moy, Peter
Project Start
1996-02-01
Project End
2008-03-31
Budget Start
2004-04-01
Budget End
2005-03-31
Support Year
9
Fiscal Year
2004
Total Cost
$413,603
Indirect Cost
Name
University of Washington
Department
Biomedical Engineering
Type
Schools of Engineering
DUNS #
605799469
City
Seattle
State
WA
Country
United States
Zip Code
98195
Jastrzebska, Katarzyna; Florczak, Anna; Kucharczyk, Kamil et al. (2018) Delivery of chemotherapeutics using spheres made of bioengineered spider silks derived from MaSp1 and MaSp2 proteins. Nanomedicine (Lond) 13:439-454
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Kirk, James T; Fridley, Gina E; Chamberlain, Jeffrey W et al. (2011) Multiplexed inkjet functionalization of silicon photonic biosensors. Lab Chip 11:1372-7
Kinahan, Michelle E; Filippidi, Emmanouela; Koster, Sarah et al. (2011) Tunable silk: using microfluidics to fabricate silk fibers with controllable properties. Biomacromolecules 12:1504-11

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