This resubmission continues the overall goal which is to develop and apply an x-ray micro-CT scanner for generating 3-D images, of sufficient spatial resolution and specimen size, to allow quantitation of intact organ microarchitecture in terms of all Basic Functional Units (BFU, e.g., glomerulus and its nephron) as well as the transport mechanisms within the organ. The application is focused on intact rodent organs or similar-sized (1-8 cm3) """"""""biopsies"""""""" from large animals and humans. In response to the previous review, more detail of scanner characteristics and how these relate to users of the scanner, are provided. The hypothesis of this proposal is that bench-top x-ray micro-CT can provide important new quantitative information about organ micro architecture by a combination of hardware and software enhancements motivated by their utility in meeting new biomedical research needs, such as defining the phenotype of genetically modified mice.
AIM I - expand the hardware and associated software to: (a) extend the capability of our current x-ray detection device with """"""""super""""""""-resolution scanning modes & reconstruction algorithms and with """"""""local"""""""" reconstruction algorithms, (b) add helical scanning capability, (c) increase x-ray flux with x-ray optics.
AIM II - Image dvnamic processes bv scanning 'snap' frozen specimen: This involves snap-freezing the specimen immediately after (a) the desired distribution of contrast agent or (b) degree of mechanical distortion (e.g., inflation) has been achieved in vivo and keeping it frozen during the scan.
AIM III - Quantitation of 3-D microarchitecture and associated 3-D function by image analysis via (a) extension of automated vascular """"""""tree"""""""" finding/analysis algorithm; (b) develop a visualization tool for guiding and evaluating the automated image analysis; (c) quantitation of local strains throughout an organ that is mechanically distorted during a scan; and (d) analysis of the fluid dynamic implications of vessel branching geometry.
AIM I V - Continue to provide the 3D micro-CT imaging service to investigator-users so that this experience will guide innovation, improvement and/or expansion of the imaging repertoire of the micro-CT methodology.

Agency
National Institute of Health (NIH)
Institute
National Institute of Biomedical Imaging and Bioengineering (NIBIB)
Type
Research Project (R01)
Project #
8R01EB000305-06
Application #
6529630
Study Section
Diagnostic Imaging Study Section (DMG)
Program Officer
Haller, John W
Project Start
1996-09-30
Project End
2005-08-31
Budget Start
2002-09-01
Budget End
2003-08-31
Support Year
6
Fiscal Year
2002
Total Cost
$390,954
Indirect Cost
Name
Mayo Clinic, Rochester
Department
Type
DUNS #
City
Rochester
State
MN
Country
United States
Zip Code
55905
Moritz, Regina; Anderson, Jill L; Vercnocke, Andrew J et al. (2013) Changes in CT angiographic opacification of porcine coronary artery wall with patchy altered flow in vasa vasorum. Int J Cardiovasc Imaging 29:1325-33
Beighley, Patricia E; Zamir, Mair; Wentz, Robert J et al. (2013) Vascularity of myocardium and gastrocnemius muscle in rats selectively bred for endurance running capacity. Physiol Genomics 45:119-25
Masyuk, Tatyana V; Radtke, Brynn N; Stroope, Angela J et al. (2013) Pasireotide is more effective than octreotide in reducing hepatorenal cystogenesis in rodents with polycystic kidney and liver diseases. Hepatology 58:409-21
Sangaralingham, S Jeson; Ritman, Erik L; McKie, Paul M et al. (2012) Cardiac micro-computed tomography imaging of the aging coronary vasculature. Circ Cardiovasc Imaging 5:518-24
Masyuk, Tatyana V; Radtke, Brynn N; Stroope, Angela J et al. (2012) Inhibition of Cdc25A suppresses hepato-renal cystogenesis in rodent models of polycystic kidney and liver disease. Gastroenterology 142:622-633.e4
Hirata, Taku; Cabrero, Pablo; Berkholz, Donald S et al. (2012) In vivo Drosophilia genetic model for calcium oxalate nephrolithiasis. Am J Physiol Renal Physiol 303:F1555-62
Thabut, Dominique; Routray, Chittaranjan; Lomberk, Gwen et al. (2011) Complementary vascular and matrix regulatory pathways underlie the beneficial mechanism of action of sorafenib in liver fibrosis. Hepatology 54:573-85
Stolz, Erwin; Yeniguen, Mesut; Kreisel, Melanie et al. (2011) Angioarchitectural changes in subacute cerebral venous thrombosis. A synchrotron-based micro- and nano-CT study. Neuroimage 54:1881-6
Kline, Timothy L; Zamir, Mair; Ritman, Erik L (2011) Relating function to branching geometry: a micro-CT study of the hepatic artery, portal vein, and biliary tree. Cells Tissues Organs 194:431-42
Liu, Baodong; Wang, Ge; Ritman, Erik L et al. (2011) Image reconstruction from limited angle projections collected by multisource interior x-ray imaging systems. Phys Med Biol 56:6337-57

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