Spider silk, which has been evolving for over 450 million years, has a tensile strength greater than steel and elasticity greater than nylon. A number of spider silk genes have been cloned and sequenced revealing specific amino acid motifs that have been conserved for over 125 million years. The key element in taking the next step toward generating bio-based materials from spider silks will be to move from the current descriptive data to predictive knowledge. These experiments will provide the predictive knowledge enabling the design of materials with very specific elastic and strength properties for each different medical application. This renewal is designed to continue testing two basic hypotheses and engineering concepts. 1) The elasticity of the materials will be proportional to the number of elastic motifs. 2) Varying the sequence of the elastic regions will vary elastic (Young's) modulus. A brief workplan is described here. 1) Continue the expression and purification of the proteins. 2) Optimize the spinning and film making process to maximize desired materials properties. 3) Test mechanical properties of films and fibers. 4) Determine the structure of the protein in films and fibers by FTIR, CD and solid state NMR. 5) The elasticity and tensile strength data will be correlated with the number and sequence of each type of motif to produce a prediction algorithm for elastic and tensile strength properties. 6) Based on 5) new genes will be constructed to match the properties needed for ligament and tendons. The past two years of work have produced a number of new genes, purified proteins and fibers from those proteins. Improvements in fiber spinning and testing of those fibers is currently in progress. This project is highly significant for several reasons. First it will provide a basic understanding of elasticity and tensile strength in spider silk proteins. Specifically, it will reveal what controls the amount of elasticity and elastic modulus and if these two factors can be varied in a predictable way. Second this project will advance our ability to use spider silk as a biomaterial. If our hypotheses are correct we will learn how to control the elasticity and other materials properties by controlling the protein sequence. Possible applications of spider silk range from artificial ligaments and tendons to bandages for burns to composite materials for multiple applications.

Agency
National Institute of Health (NIH)
Institute
National Institute of Biomedical Imaging and Bioengineering (NIBIB)
Type
Research Project (R01)
Project #
5R01EB000490-07
Application #
7623848
Study Section
Biomaterials and Biointerfaces Study Section (BMBI)
Program Officer
Henderson, Lori
Project Start
2002-07-01
Project End
2011-05-31
Budget Start
2009-06-01
Budget End
2011-05-31
Support Year
7
Fiscal Year
2009
Total Cost
$331,483
Indirect Cost
Name
University of Wyoming
Department
Biochemistry
Type
Schools of Earth Sciences/Natur
DUNS #
069690956
City
Laramie
State
WY
Country
United States
Zip Code
82071
Albertson, Amy E; Teulé, Florence; Weber, Warner et al. (2014) Effects of different post-spin stretching conditions on the mechanical properties of synthetic spider silk fibers. J Mech Behav Biomed Mater 29:225-34
Adrianos, Sherry L; Teulé, Florence; Hinman, Michael B et al. (2013) Nephila clavipes Flagelliform silk-like GGX motifs contribute to extensibility and spacer motifs contribute to strength in synthetic spider silk fibers. Biomacromolecules 14:1751-60
Teulé, Florence; Addison, Bennett; Cooper, Alyssa R et al. (2012) Combining flagelliform and dragline spider silk motifs to produce tunable synthetic biopolymer fibers. Biopolymers 97:418-31
An, Bo; Jenkins, Janelle E; Sampath, Sujatha et al. (2012) Reproducing natural spider silks' copolymer behavior in synthetic silk mimics. Biomacromolecules 13:3938-48
An, Bo; Hinman, Michael B; Holland, Gregory P et al. (2011) Inducing ?-sheets formation in synthetic spider silk fibers by aqueous post-spin stretching. Biomacromolecules 12:2375-81
Creager, Melinda S; Izdebski, Thomas; Brooks, Amanda E et al. (2011) Elucidating metabolic pathways for amino acid incorporation into dragline spider silk using 13C enrichment and solid state NMR. Comp Biochem Physiol A Mol Integr Physiol 159:219-24
Holland, Gregory P; Cherry, Brian R; Jenkins, Janelle E et al. (2010) Proton-detected heteronuclear single quantum correlation NMR spectroscopy in rigid solids with ultra-fast MAS. J Magn Reson 202:64-71
Creager, Melinda S; Jenkins, Janelle E; Thagard-Yeaman, Leigh A et al. (2010) Solid-state NMR comparison of various spiders' dragline silk fiber. Biomacromolecules 11:2039-43
Jenkins, Janelle E; Creager, Melinda S; Butler, Emily B et al. (2010) Solid-state NMR evidence for elastin-like beta-turn structure in spider dragline silk. Chem Commun (Camb) 46:6714-6
Perry, David J; Bittencourt, Daniela; Siltberg-Liberles, Jessica et al. (2010) Piriform spider silk sequences reveal unique repetitive elements. Biomacromolecules 11:3000-6

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